Human PIP4K2A protein (Recombinant) (C-His) (STJP013343)
SPECIFICATIONS
HostMammalian Cells
ConjugationUnconjugated
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human PIP4K2A-C-His protein was developed from mammalian cells and has a target region of C-His. For use in research applications. |
| Applications | ELISA/WB |
| Host | Mammalian Cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Reconstitute in sterile water for a stock solution. |
| Formulation | Lyophilized from a 0.22 Mu m filtered solution in 20mM PB, 150mM NaCl, pH 7.4. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | < 1 EU/µg as determined by LAL test. |
Target Information
| Gene Symbol | PIP4K2A |
| Gene ID | 5305 |
| Uniprot ID | PI42A_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Met1-Thr406 |
Additional Info
| Post Translational Modifications | Phosphorylated in tyrosines. Phosphorylation is induced by light and increases kinase activity. |
| Function | Catalyzes the phosphorylation of phosphatidylinositol 5-phosphate (PtdIns5P) on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Has both ATP- and GTP-dependent kinase activities. May exert its function by regulating the levels of PtdIns5P, which functions in the cytosol by increasing AKT activity and in the nucleus signals through ING2. May regulate the pool of cytosolic PtdIns5P in response to the activation of tyrosine phosphorylation. Required for lysosome-peroxisome membrane contacts and intracellular cholesterol transport through modulating peroxisomal PtdIns(4,5)P2 level. In collaboration with PIP4K2B, has a role in mediating autophagy in times of nutrient stress. Required for autophagosome-lysosome fusion and the regulation of cellular lipid metabolism. May be involved in thrombopoiesis, and the terminal maturation of megakaryocytes and regulation of their size. Negatively regulates insulin signaling through a catalytic-independent mechanism. PIP4Ks interact with PIP5Ks and suppress PIP5K-mediated PtdIns(4,5)P2 synthesis and insulin-dependent conversion to PtdIns(3,4,5)P3. |
| Protein Name | Phosphatidylinositol 5-Phosphate 4-Kinase Type-2 Alpha1-Phosphatidylinositol 5-Phosphate 4-Kinase 2-AlphaDiphosphoinositide Kinase 2-AlphaPip5kiiiPhosphatidylinositol 5-Phosphate 4-KinasePi5p4kalphaPhosphatidylinositol 5-Phosphate 4-Kinase Type Ii AlphaPi(5p 4-Kinase Type Ii AlphaPip4kii-AlphaPtdins(4p-5-Kinase B IsoformPtdins(4p-5-Kinase C IsoformPtdins(5p-4-Kinase Isoform 2-Alpha |
| Database Links | Reactome: R-HSA-1660499Reactome: R-HSA-6811555Reactome: R-HSA-6811558Reactome: R-HSA-8847453 |
| Cellular Localisation | Cell MembraneNucleusLysosomeCytoplasmPhotoreceptor Inner SegmentCell ProjectionCiliumPhotoreceptor Outer SegmentMay Translocate From The Cytosol To The Cell Membrane Upon Activation Of Tyrosine PhosphorylationMay Translocate From The Inner To The Outer Segments Of The Rod Photoreceptor Cells In Response To LightLocalization To The Nucleus Is Modulated By The Interaction With Pip4k2b |
| Alternative Protein Names | Phosphatidylinositol 5-Phosphate 4-Kinase Type-2 Alpha protein1-Phosphatidylinositol 5-Phosphate 4-Kinase 2-Alpha proteinDiphosphoinositide Kinase 2-Alpha proteinPip5kiii proteinPhosphatidylinositol 5-Phosphate 4-Kinase proteinPi5p4kalpha proteinPhosphatidylinositol 5-Phosphate 4-Kinase Type Ii Alpha proteinPi(5p 4-Kinase Type Ii Alpha proteinPip4kii-Alpha proteinPtdins(4p-5-Kinase B Isoform proteinPtdins(4p-5-Kinase C Isoform proteinPtdins(5p-4-Kinase Isoform 2-Alpha proteinPIP4K2A proteinPI5P4KA proteinPIP5K2 proteinPIP5K2A protein |
Information sourced from Uniprot.org