Human PIK3C2A protein (Recombinant) (N-His) (STJP003152)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human PIK3C2A-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | PIK3C2A |
| Gene ID | 5286 |
| Uniprot ID | P3C2A_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Phe172-Asn403 |
Additional Info
| Post Translational Modifications | Phosphorylated upon insulin stimulation.which may lead to enzyme activation. Phosphorylated on Ser-259 during mitosis and upon UV irradiation.which does not change enzymatic activity but leads to proteasomal degradation. Ser-259 phosphorylation may be mediated by CDK1 or JNK, depending on the physiological state of the cell. |
| Function | Generates phosphatidylinositol 3-phosphate (PtdIns3P) and phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2) that act as second messengers. Has a role in several intracellular trafficking events. Functions in insulin signaling and secretion. Required for translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane and glucose uptake in response to insulin-mediated RHOQ activation. Regulates insulin secretion through two different mechanisms: involved in glucose-induced insulin secretion downstream of insulin receptor in a pathway that involves AKT1 activation and TBC1D4/AS160 phosphorylation, and participates in the late step of insulin granule exocytosis probably in insulin granule fusion. Synthesizes PtdIns3P in response to insulin signaling. Functions in clathrin-coated endocytic vesicle formation and distribution. Regulates dynamin-independent endocytosis, probably by recruiting EEA1 to internalizing vesicles. In neurosecretory cells synthesizes PtdIns3P on large dense core vesicles. Participates in calcium induced contraction of vascular smooth muscle by regulating myosin light chain (MLC) phosphorylation through a mechanism involving Rho kinase-dependent phosphorylation of the MLCP-regulatory subunit MYPT1. May play a role in the EGF signaling cascade. May be involved in mitosis and UV-induced damage response. Required for maintenance of normal renal structure and function by supporting normal podocyte function. Involved in the regulation of ciliogenesis and trafficking of ciliary components. |
| Protein Name | Phosphatidylinositol 4-Phosphate 3-Kinase C2 Domain-Containing Subunit AlphaPi3k-C2-AlphaPtdins-3-Kinase C2 Subunit Alpha Phosphoinositide 3-Kinase-C2-Alpha |
| Database Links | Reactome: R-HSA-1660499Reactome: R-HSA-1660514Reactome: R-HSA-1660516Reactome: R-HSA-1660517Reactome: R-HSA-432722Reactome: R-HSA-8856828 |
| Cellular Localisation | Cell MembraneCytoplasmic VesicleClathrin-Coated VesicleNucleusCytoplasmGolgi ApparatusTrans-Golgi NetworkInserts Preferentially Into Membranes Containing Ptdins(45)P2Associated With Rna-Containing Structures |
| Alternative Protein Names | Phosphatidylinositol 4-Phosphate 3-Kinase C2 Domain-Containing Subunit Alpha proteinPi3k-C2-Alpha proteinPtdins-3-Kinase C2 Subunit Alpha proteinPhosphoinositide 3-Kinase-C2-Alpha proteinPIK3C2A protein |
Information sourced from Uniprot.org