Human PHLPP1 protein (Recombinant) (N-His) (STJP007493)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human PHLPP1-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | PHLPP1 |
| Gene ID | 23239 |
| Uniprot ID | PHLP1_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Ser1168-Thr1425 |
Additional Info
| Function | Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family.specifically acts on 'Ser-473' of AKT2 and AKT3, 'Ser-660' of PRKCB and 'Ser-657' of PRKCA. Isoform 2 seems to have a major role in regulating Akt signaling in hippocampal neurons. Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and suppression of tumor growth. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis. Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation. Inhibits cancer cell proliferation and may act as a tumor suppressor. Dephosphorylates RAF1 inhibiting its kinase activity. May act as a negative regulator of K-Ras signaling in membrane rafts. Involved in the hippocampus-dependent long-term memory formation. Involved in circadian control by regulating the consolidation of circadian periodicity after resetting. Involved in development and function of regulatory T-cells. |
| Protein Name | Ph Domain Leucine-Rich Repeat-Containing Protein Phosphatase 1Pleckstrin Homology Domain-Containing Family E Member 1Ph Domain-Containing Family E Member 1Suprachiasmatic Nucleus Circadian Oscillatory ProteinHscop |
| Database Links | Reactome: R-HSA-199418 |
| Cellular Localisation | CytoplasmMembranePeripheral Membrane ProteinNucleusIn Colorectal Cancer TissueExpression Is Concentrated At The Lateral Membrane Of Epithelial CellsIsoform 2: Cell Membrane |
| Alternative Protein Names | Ph Domain Leucine-Rich Repeat-Containing Protein Phosphatase 1 proteinPleckstrin Homology Domain-Containing Family E Member 1 proteinPh Domain-Containing Family E Member 1 proteinSuprachiasmatic Nucleus Circadian Oscillatory Protein proteinHscop proteinPHLPP1 proteinKIAA0606 proteinPHLPP proteinPLEKHE1 proteinSCOP protein |
Information sourced from Uniprot.org