Human PHF2 protein (Recombinant) (N-His) (STJP016314)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human PHF2-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | Please contact us for further information. |
Target Information
| Gene Symbol | PHF2 |
| Gene ID | 5253 |
| Uniprot ID | PHF2_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Gln83-Arg450 |
Additional Info
| Post Translational Modifications | Phosphorylated by PKA on specific serine residues, leading to the formation of an active lysine demethylase complex. |
| Function | Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA. Involved in the activation of toll-like receptor 4 (TLR4)-target inflammatory genes in macrophages by catalyzing the demethylation of trimethylated histone H4 lysine 20 (H4K20me3) at the gene promoters. |
| Protein Name | Lysine-Specific Demethylase Phf2Grc5Phd Finger Protein 2 |
| Database Links | Reactome: R-HSA-3214842 |
| Cellular Localisation | NucleusNucleolusChromosomeCentromereKinetochore |
| Alternative Protein Names | Lysine-Specific Demethylase Phf2 proteinGrc5 proteinPhd Finger Protein 2 proteinPHF2 proteinCENP-35 proteinKIAA0662 protein |
Information sourced from Uniprot.org