Human Peroxiredoxin 2/PRDX2 protein (Recombinant) (No-Tag) (STJP016419)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human Peroxiredoxin 2/PRDX2-No-Tag protein was developed from e.coli and has a target region of No-Tag. For use in research applications. |
| Applications | SDS-PAGE/Enzyme Activity |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 1 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 10% Glycerol |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Endotoxin | < 1 EU per 1ug of protein (determined by LAL method) |
| Immunoreactivity | Specific activity is >2, 000pmol/min/ug. Enzymatic activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25C for minute. |
Target Information
| Gene Symbol | PRDX2 |
| Gene ID | 7001 |
| Uniprot ID | PRDX2_HUMAN |
| Accession Number | NP_005800.3 |
| Immunogen | Human |
| Immunogen Region | 1-198aa |
| Immunogen Sequence | MASGNARIGK PAPDFKATAV VDGAFKEVKL SDYKGKYVVL FFYPLDFTFV CPTEIIAFSN RAEDFRKLGC EVLGVSVDSQ FTHLAWINTP RKEGGLGPLN IPLLADVTRR LSEDYGVLKT DEGIAYRGLF IIDGKGVLRQ ITVNDLPVGR SVDEALRLVQ AFQYTDEHGE VCPAGWKPGS DTIKPNVDDS KEYFSKHN |
Additional Info
| Post Translational Modifications | The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) instead of its condensation to a disulfide bond. It can be reactivated by forming a transient disulfide bond with sulfiredoxin SRXN1, which reduces the cysteine sulfinic acid in an ATP- and Mg-dependent manner. Acetylation increases resistance to transition to high molecular-mass complexes. Deacetylated by HDAC6 which decreases reducing activity. |
| Function | Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). |
| Protein Name | Peroxiredoxin-2Natural Killer Cell-Enhancing Factor BNkef-BPrpThiol-Specific Antioxidant ProteinTsaThioredoxin Peroxidase 1Thioredoxin-Dependent Peroxide Reductase 1Thioredoxin-Dependent Peroxiredoxin 2 |
| Database Links | Reactome: R-HSA-3299685Reactome: R-HSA-5628897Reactome: R-HSA-8862803 |
| Cellular Localisation | Cytoplasm |
| Alternative Protein Names | Peroxiredoxin-2 proteinNatural Killer Cell-Enhancing Factor B proteinNkef-B proteinPrp proteinThiol-Specific Antioxidant Protein proteinTsa proteinThioredoxin Peroxidase 1 proteinThioredoxin-Dependent Peroxide Reductase 1 proteinThioredoxin-Dependent Peroxiredoxin 2 proteinPRDX2 proteinNKEFB proteinTDPX1 protein |
Information sourced from Uniprot.org