Human PDGFR beta (PDGFR beta) Antibody Pair (STJAP00810)
General Information
| Short Description | This PDGFR beta antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R11-4M-8/R39-2X-5 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | PDGFRB |
| Gene ID | 5159 |
| Uniprot ID | PGFRB_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Post Translational Modifications | Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-579, and to a lesser degree, at Tyr-581, is important for interaction with SRC family kinases. Phosphorylation at Tyr-740 and Tyr-751 is important for interaction with PIK3R1. Phosphorylation at Tyr-751 is important for interaction with NCK1. Phosphorylation at Tyr-771 and Tyr-857 is important for interaction with RASA1/GAP. Phosphorylation at Tyr-857 is important for efficient phosphorylation of PLCG1 and PTPN11, resulting in increased phosphorylation of AKT1, MAPK1/ERK2 and/or MAPK3/ERK1, PDCD6IP/ALIX and STAM, and in increased cell proliferation. Phosphorylation at Tyr-1009 is important for interaction with PTPN11. Phosphorylation at Tyr-1009 and Tyr-1021 is important for interaction with PLCG1. Phosphorylation at Tyr-1021 is important for interaction with CBL.PLCG1 and CBL compete for the same binding site. Dephosphorylated by PTPRJ at Tyr-751, Tyr-857, Tyr-1009 and Tyr-1021. Dephosphorylated by PTPN2 at Tyr-579 and Tyr-1021. N-glycosylated. Ubiquitinated. After autophosphorylation, the receptor is polyubiquitinated, leading to its degradation. |
| Function | Tyrosine-protein kinase that acts as a cell-surface receptor for homodimeric PDGFB and PDGFD and for heterodimers formed by PDGFA and PDGFB, and plays an essential role in the regulation of embryonic development, cell proliferation, survival, differentiation, chemotaxis and migration. Plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites. Required for normal development of the cardiovascular system. Required for normal recruitment of pericytes (mesangial cells) in the kidney glomerulus, and for normal formation of a branched network of capillaries in kidney glomeruli. Promotes rearrangement of the actin cytoskeleton and the formation of membrane ruffles. Binding of its cognate ligands - homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFD -leads to the activation of several signaling cascades.the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PLCG1, PIK3R1, PTPN11, RASA1/GAP, CBL, SHC1 and NCK1. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to the activation of the AKT1 signaling pathway. Phosphorylation of SHC1, or of the C-terminus of PTPN11, creates a binding site for GRB2, resulting in the activation of HRAS, RAF1 and down-stream MAP kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation and activation of SRC family kinases. Promotes phosphorylation of PDCD6IP/ALIX and STAM. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor. |
| Protein Name | Platelet-Derived Growth Factor Receptor BetaPdgf-R-BetaPdgfr-BetaBeta Platelet-Derived Growth Factor ReceptorBeta-Type Platelet-Derived Growth Factor ReceptorCd140 Antigen-Like Family Member BPlatelet-Derived Growth Factor Receptor 1Pdgfr-1Cd Antigen Cd140b |
| Database Links | Reactome: R-HSA-1257604Reactome: R-HSA-186763Reactome: R-HSA-186797Reactome: R-HSA-2219530Reactome: R-HSA-5673001Reactome: R-HSA-6811558 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCytoplasmic VesicleLysosome LumenAfter Ligand BindingThe Autophosphorylated Receptor Is Ubiquitinated And InternalizedLeading To Its Degradation |
| Alternative Antibody Pair Names | Platelet-Derived Growth Factor Receptor Beta antibody pairPdgf-R-Beta antibody pairPdgfr-Beta antibody pairBeta Platelet-Derived Growth Factor Receptor antibody pairBeta-Type Platelet-Derived Growth Factor Receptor antibody pairCd140 Antigen-Like Family Member B antibody pairPlatelet-Derived Growth Factor Receptor 1 antibody pairPdgfr-1 antibody pairCd Antigen Cd140b antibody pairPDGFRB antibody pairPDGFR antibody pairPDGFR1 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human PDGFR beta (PDGFR beta) Antibody Pair (STJAP00810)
This antibody pair is designed to detect human PDGFR beta (UniProt P09619) and has confirmed reactivity against human samples only.
This antibody pair has been validated for use in ELISA. It is intended for research use only and is not validated for diagnostic or therapeutic applications.
Both antibodies are supplied as unconjugated, affinity-purified rabbit monoclonal IgGs at 1 mg/mL, formulated in 0.01M PBS at pH 7.2. The clone IDs are R11-4M-8 and R39-2X-5.
Optimal working concentrations for both the capture and detection antibodies are to be determined by the end user, as no predefined dilution range is specified for this product.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquot and store at -20°C, and avoid repeated freeze/thaw cycles to maintain activity.
Both antibodies were raised in rabbit against a recombinant protein immunogen corresponding to human PDGFR beta. No specific immunogen region is defined in the product documentation.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.