This human PDE4D kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
Applications
CLIA
Reactivity
Human
Sensitivity
37.5pg/mL
Detection Limit
62.5~4000pg/mL
Note
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Product Properties
Storage Instruction
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
This kit recognizes Human PDE4D in samples. No significant cross-reactivity or interference between Human PDE4D and analogues was observed.
Sample Type
Serum, plasma and other biological fluids
Additional Info
Tissue Specificity
Expressed in colonic epithelial cells (at protein level). Widespread.most abundant in skeletal muscle. Isoform 6: Detected in brain. Isoform 8: Detected in brain, placenta, lung and kidney. Isoform 7: Detected in heart and skeletal muscle.
Post Translational Modifications
Long isoforms that share a conserved PKA phosphorylation site in the N-terminus are activated by PKA through phosphorylation. Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59. Sumoylation of long isoforms by PIAS4 augments their activation by PKA phosphorylation and represses their inhibition by ERK phosphorylation.
Function
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
Apical Cell MembraneCytoplasmMembraneCytoskeletonMicrotubule Organizing CenterCentrosomeFound In The Soluble FractionAssociated With MembranesAnd Associated With The Cytoskeleton And The CentrosomeColocalized With Shank2 To The Apical Membrane Of Colonic Crypt Cells
