Human PD-1 (Programmed Cell Death Protein 1) Antibody Pair (STJAP00431)
General Information
| Short Description | This PD-1 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Mouse |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Protein A or G. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | PDCD1 |
| Gene ID | 5133 |
| Uniprot ID | PDCD1_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human PD-1 protien.Detection Immunogen: Recombinant Human PD-1 protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Function | Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2. Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation. Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta. The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival. The interaction with CD274/PDCD1L1 inhibits cytotoxic T lymphocytes (CTLs) effector function. The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy. |
| Protein Name | Programmed Cell Death Protein 1Protein Pd-1Hpd-1Cd Antigen Cd279 |
| Database Links | Reactome: R-HSA-389948Reactome: R-HSA-9679191 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Pair Names | Programmed Cell Death Protein 1 antibody pairProtein Pd-1 antibody pairHpd-1 antibody pairCd Antigen Cd279 antibody pairPDCD1 antibody pairPD1 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human PD-1 (Programmed Cell Death Protein 1) Antibody Pair (STJAP00431)
This antibody pair detects Human PD-1 (Programmed Cell Death Protein 1, UniProt Q15116) and is validated for human reactivity only. Both the capture and detection antibodies were raised against recombinant human PD-1 protein.
The capture antibody is unconjugated and supplied in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.5, while the detection antibody is biotinylated and supplied in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol at pH 7.5. Both components are provided at a concentration of 0.5 mg/mL and were purified using Protein A or G.
This antibody pair is validated for use in ELISA. It is intended for research use only and is not validated for diagnostic or therapeutic applications.
This antibody pair is suitable for use with serum, plasma, and other biological fluids. Researchers should optimize assay conditions for sample types not explicitly listed.
Both components should be stored at -20°C for long-term storage. Repeated freeze-thaw cycles should be avoided to maintain antibody integrity.
Both the capture and detection antibodies are mouse-derived, so assay development should include appropriate blocking steps or the use of anti-mouse secondary reagents that do not cross-react with the capture antibody. No specific clone IDs or isotype information is provided for this pair, so researchers should empirically validate the assay setup to minimize background from anti-mouse detection reagents.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.