• 96-well maxisorp plates were coated at 1ug/well recombinant human PD-L1 Then, the protein standard was added, and plates were incubated for 2 hrs at 37°C. Absorbance was measured at 450-615 nm using an automated plate reader.

Human PD-1 (PDCD1) protein (Recombinant) {Biotin} (STJP000210)

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STJP000210

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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human PD-1 (PDCD1)-protein was developed from hek293. For use in research applications.
Conjugation: Biotin
Formulation: Lyophilised from 0.2 Mu m filtered PBS solution, pH7.2, 5% Trehalose.
Dilution Range: A quick spin of the vial followed by reconstitution in sterile PBS pH 7.4 to a concentration not less than 0.2 mg/mL. Solubilize for 1hr at room temp with gentle mixing.
Storage Instruction: Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year.It is recommended to add 0.1% HSA or BSA for long term storage. Avoid repeated freeze/thaw cycles. NA
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA
Immunoreactivity: The activity was tested by the ability of immobilized recombinant Human PD-1 to bind recombinant human PD-L1 within a linear range of 0.1-5.0 ng/ml in ELISA. NA
Gene Symbol: PDCD1
Gene ID: 5133
Uniprot ID: PDCD1_HUMAN
Immunogen Region: PRO21-GLN167, ECD
Immunogen: Optimized DNA sequence encoding extracellular domain of Human Programmed cell death protein 1 PD-1 (PRO21-GLN167) including a C-terminal His tag was expressed in HEK293 cells. NA
Post Translational Modifications Ubiquitinated at Lys-233 by the SCF(FBXO38) complex, leading to its proteasomal degradation. Ubiquitinated via 'Lys-48'-linked polyubiquitin chains. Tyrosine phosphorylated at Tyr-223 (within ITIM motif) and Tyr-248 (ITSM motif) upon ligand binding. Phosphorylation at Tyr-248 promotes the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta. N-glycosylation at Asn-58 contains at least two N-acetylglucosamine units and one fucose. N-glycosylation does not affect binding to nivolumab drug.
Function Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2. Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation. Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta. The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival. The interaction with CD274/PDCD1L1 inhibits cytotoxic T lymphocytes (CTLs) effector function. The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy.
Protein Name Programmed Cell Death Protein 1
Protein Pd-1
Hpd-1
Cd Antigen Cd279
Database Links Reactome: R-HSA-389948
Reactome: R-HSA-9679191
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Protein Names Programmed Cell Death Protein 1 protein
Protein Pd-1 protein
Hpd-1 protein
Cd Antigen Cd279 protein
PDCD1 protein
PD1 protein

Information sourced from Uniprot.org

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