Human PCX (Podocalyxin) Antibody Pair (STJAP00430)
General Information
| Short Description | This PCX antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Goat/Goat |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | PODXL |
| Gene ID | 5420 |
| Uniprot ID | PODXL_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human PCX protien.Detection Immunogen: Recombinant Human PCX protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | N- and O-linked glycosylated. Sialoglycoprotein. |
| Function | Involved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells. |
| Protein Name | PodocalyxinGctm-2 AntigenGp200Podocalyxin-Like Protein 1PcPclp-1 |
| Database Links | |
| Cellular Localisation | Apical Cell MembraneCell ProjectionLamellipodiumFilopodiumRuffleMicrovillusMembrane RaftMembraneSingle-Pass Type I Membrane ProteinIn Single Attached Epithelial Cells Is Restricted To A Preapical Pole On The Free Plasma Membrane Whereas Other Apical And Basolateral Proteins Are Not Yet PolarizedColocalizes With Nherf2 At The Apical Plasma Membrane During Epithelial PolarizationColocalizes With Nherf1 At The Trans-Golgi Network (Transiently) And At The Apical Plasma MembraneIts Association With The Membrane Raft Is TransientColocalizes With Actin FilamentsEzr And Nherf1 In A Punctate Pattern At The Apical Cell Surface Where Microvilli FormColocalizes With Ezr And Nherf2 At The Apical Cell Membrane Of Glomerular Epithelium CellsForms GranularPunctuated PatternForming PatchesPreferentially Adopting A Polar DistributionLocated On The Migrating Poles Of The Cell Or Forming Clusters Along The Terminal Ends Of Filipodia Establishing Contact With The Endothelial CellsColocalizes With The Submembrane Actin Of LamellipodiaParticularly Associated With RufflesColocalizes With Vinculin At Protrusions Of CellsColocalizes With Itgb1Colocalizes With Pard3PrkciExoc5OclnRab11a And Rab8a In Apical Membrane Initiation Sites (Amis) During The Generation Of Apical Surface And Luminogenesis |
| Alternative Antibody Pair Names | Podocalyxin antibody pairGctm-2 Antigen antibody pairGp200 antibody pairPodocalyxin-Like Protein 1 antibody pairPc antibody pairPclp-1 antibody pairPODXL antibody pairPCLP antibody pairPCLP1 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human PCX (Podocalyxin) Antibody Pair (STJAP00430)
This antibody pair detects human Podocalyxin (PCX), corresponding to UniProt entry O00592. Reactivity is confirmed for human samples only.
The capture antibody is unconjugated and the detection antibody is biotinylated. Both are goat-derived, antigen affinity-purified, and supplied at 0.5 mg/mL.
This pair has been validated for ELISA. No other assay formats are specified for this product.
This pair is intended for use with serum, plasma, and other biological fluids. It is for research use only and is not validated for diagnostic applications.
Both the capture and detection antibodies should be stored at -20 degrees Celsius for long-term storage, and freeze-thaw cycles should be avoided. The capture antibody is formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.5, while the detection antibody is formulated in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol at pH 7.5.
Both the capture and detection antibodies were raised against recombinant human PCX protein. Both antibodies were subsequently purified by antigen affinity chromatography.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.