Human PCLAF protein (Recombinant) (N-His) (STJP006667)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human PCLAF-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | PCLAF |
| Gene ID | 9768 |
| Uniprot ID | PAF15_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Met1-Glu111 |
Additional Info
| Post Translational Modifications | Monoubiquitinated at Lys-15 and Lys-24 during normal S phase, promoting its association with PCNA. Also diubiquitinated at these 2 sites. Following DNA damage, monoubiquitin chains at Lys-15 and Lys-24 are probably extended, leading to disrupt the interaction with PCNA. Polyubiquitinated by the APC/C complex at the mitotic exit, leading to its degradation by the proteasome. |
| Function | PCNA-binding protein that acts as a regulator of DNA repair during DNA replication. Following DNA damage, the interaction with PCNA is disrupted, facilitating the interaction between monoubiquitinated PCNA and the translesion DNA synthesis DNA polymerase eta (POLH) at stalled replisomes, facilitating the bypass of replication-fork-blocking lesions. Also acts as a regulator of centrosome number. |
| Protein Name | Pcna-Associated FactorHepatitis C Virus Ns5a-Transactivated Protein 9Hcv Ns5a-Transactivated Protein 9Overexpressed In Anaplastic Thyroid Carcinoma 1Oeatc-1Pcna-Associated Factor Of 15 KdaPaf15P15pafPcna-Clamp-Associated Factor |
| Database Links | Reactome: R-HSA-5656169 |
| Cellular Localisation | NucleusCytoplasmPerinuclear RegionFollowing Dna DamageLocalizes To Dna Damage SitesColocalizes With Centrosomes In Perinuclear Region |
| Alternative Protein Names | Pcna-Associated Factor proteinHepatitis C Virus Ns5a-Transactivated Protein 9 proteinHcv Ns5a-Transactivated Protein 9 proteinOverexpressed In Anaplastic Thyroid Carcinoma 1 proteinOeatc-1 proteinPcna-Associated Factor Of 15 Kda proteinPaf15 proteinP15paf proteinPcna-Clamp-Associated Factor proteinPCLAF proteinKIAA0101 proteinNS5ATP9 proteinPAF proteinL5 protein |
Information sourced from Uniprot.org