Human PAPPA (Pappalysin-1) CLIA Kit for Quantitative Detection

Name: Human PAPPA (Pappalysin-1) CLIA Kit for Quantitative Detection
SKU: STJC0000118
Availability: InStock

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SPECIFICATIONS

STJC0000118

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Short Description	This human PAPPA kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
Applications	CLIA
Reactivity	Human
Sensitivity	0.09ng/mL
Detection Limit	0.16~10ng/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Chemiluminescence

Gene Symbol	PAPPA
Gene ID	5069
Uniprot ID	PAPP1_HUMAN
Specificity	This kit recognizes Human PAPPA in samples. No significant cross-reactivity or interference between Human PAPPA and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Tissue Specificity	High levels in placenta and pregnancy serum. In placenta, expressed in X cells in septa and anchoring villi, and in syncytiotrophoblasts in the chorionic villi. Lower levels are found in a variety of other tissues including kidney, myometrium, endometrium, ovaries, breast, prostate, bone marrow, colon, fibroblasts and osteoblasts.
Post Translational Modifications	There appear to be no free sulfhydryl groups.
Function	Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF.
Protein Name	Pappalysin-1 Insulin-Like Growth Factor-Dependent Igf-Binding Protein 4 Protease Igf-Dependent Igfbp-4 Protease Igfbp-4ase Pregnancy-Associated Plasma Protein A Papp-A
Database Links	Reactome: R-HSA-381426
Cellular Localisation	Secreted
Alternative CLIA Names	Pappalysin-1 CLIA kit Insulin-Like Growth Factor-Dependent Igf-Binding Protein 4 Protease CLIA kit Igf-Dependent Igfbp-4 Protease CLIA kit Igfbp-4ase CLIA kit Pregnancy-Associated Plasma Protein A CLIA kit Papp-A CLIA kit PAPPA CLIA kit
Specificity	This kit recognizes Human PAPPA in samples. No significant cross-reactivity or interference between Human PAPPA and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	0.50	1.46	4.91	0.51	1.37	4.54
Standard deviation	0.05	0.17	0.36	0.05	0.11	0.51
CV (%)	10.00	11.64	7.33	9.80	8.03	11.23