Human PAPPA (Pappalysin-1) Antibody Pair (STJAP00131)
General Information
| Short Description | This PAPPA antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Mouse |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Protein A or G. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | PAPPA |
| Gene ID | 5069 |
| Uniprot ID | PAPP1_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human PAPPA protein.Detection Immunogen: Recombinant Human PAPPA protein. |
Additional Info
| Post Translational Modifications | There appear to be no free sulfhydryl groups. |
| Function | Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF. |
| Protein Name | Pappalysin-1Insulin-Like Growth Factor-Dependent Igf-Binding Protein 4 ProteaseIgf-Dependent Igfbp-4 ProteaseIgfbp-4asePregnancy-Associated Plasma Protein APapp-A |
| Database Links | Reactome: R-HSA-381426 |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | Pappalysin-1 antibody pairInsulin-Like Growth Factor-Dependent Igf-Binding Protein 4 Protease antibody pairIgf-Dependent Igfbp-4 Protease antibody pairIgfbp-4ase antibody pairPregnancy-Associated Plasma Protein A antibody pairPapp-A antibody pairPAPPA antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human PAPPA (Pappalysin-1) Antibody Pair (STJAP00131)
This antibody pair detects human PAPPA (Pappalysin-1), corresponding to UniProt entry Q13219. Reactivity is confirmed for human samples only.
Both antibodies are mouse monoclonal antibodies supplied at 0.5 mg/mL. The capture antibody is unconjugated, while the detection antibody is biotinylated. Both are formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.4.
This antibody pair is validated for use in ELISA. No other assay formats are listed in the product specifications.
Both the capture and detection antibodies were purified using Protein A or Protein G affinity chromatography. Both were raised against recombinant human PAPPA protein as the immunogen.
Both components should be stored at -20 degrees Celsius for long-term storage. Freeze-thaw cycles should be avoided to maintain antibody integrity.
The product specifications list reactivity for human only. No cross-reactivity data for other species is provided, and researchers requiring cross-species detection should verify suitability through independent testing.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.