| Applications: | ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 13.5pg/mL |
| Detection Limit: | 31.2-2000pg/mL |
| Short Description: | This PAM Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma, tissue homogenates or other biological fluids.. |
| Storage Instruction: | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 4.5 hrs |
| Gene Symbol: | PAM |
| Gene ID: | 5066 |
| Uniprot ID: | AMD_HUMAN |
| Sample Type: | serum, plasma, tissue homogenates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | |
| Function | Bifunctional enzyme that catalyzes the post-translational modification of inactive peptidylglycine precursors to the corresponding bioactive alpha-amidated peptides, a terminal modification in biosynthesis of many neural and endocrine peptides. Alpha-amidation involves two sequential reactions, both of which are catalyzed by separate catalytic domains of the enzyme. The first step, catalyzed by peptidyl alpha-hydroxylating monooxygenase (PHM) domain, is the copper-, ascorbate-, and O2- dependent stereospecific hydroxylation (with S stereochemistry) at the alpha-carbon (C-alpha) of the C-terminal glycine of the peptidylglycine substrate. The second step, catalyzed by the peptidylglycine amidoglycolate lyase (PAL) domain, is the zinc-dependent cleavage of the N-C-alpha bond, producing the alpha-amidated peptide and glyoxylate. Similarly, catalyzes the two-step conversion of an N-fatty acylglycine to a primary fatty acid amide and glyoxylate. |
| Protein Name | Peptidyl-Glycine Alpha-Amidating Monooxygenase Pam Includes - Peptidylglycine Alpha-Hydroxylating Monooxygenase Phm - Peptidyl-Alpha-Hydroxyglycine Alpha-Amidating Lyase Peptidylamidoglycolate Lyase Pal |
| Database Links | |
| Cellular Localisation | Cytoplasmic Vesicle Secretory Vesicle Membrane Single-Pass Membrane Protein Secretory Granules Isoform 1: Membrane Single-Pass Type I Membrane Protein Isoform 2: Membrane Isoform 3: Secreted Secreted From Secretory Granules Isoform 4: Secreted |
| Alternative ELISA Names | Peptidyl-Glycine Alpha-Amidating Monooxygenase ELISA kit Pam Includes - Peptidylglycine Alpha-Hydroxylating Monooxygenase ELISA kit Phm - Peptidyl-Alpha-Hydroxyglycine Alpha-Amidating Lyase ELISA kit Peptidylamidoglycolate Lyase ELISA kit Pal ELISA kit PAM ELISA kit |
| output |
Information sourced from Uniprot.org
