Human PADI4 protein (Recombinant) (N-His) (STJP006202)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human PADI4-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | PADI4 |
| Gene ID | 23569 |
| Uniprot ID | PADI4_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Met1-Pro300 |
Additional Info
| Post Translational Modifications | Autocitrullination at Arg-372 and Arg-374 inactivates the enzyme. |
| Function | Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci. Required for the formation of neutrophil extracellular traps (NETs).NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation. Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1. |
| Protein Name | Protein-Arginine Deiminase Type-4Hl-60 PadPeptidylarginine Deiminase IvProtein-Arginine Deiminase Type Iv |
| Database Links | Reactome: R-HSA-3247509 |
| Cellular Localisation | CytoplasmNucleusCytoplasmic GranuleCytoplasmic Granules Of Eosinophils And Neutrophils |
| Alternative Protein Names | Protein-Arginine Deiminase Type-4 proteinHl-60 Pad proteinPeptidylarginine Deiminase Iv proteinProtein-Arginine Deiminase Type Iv proteinPADI4 proteinPAD4 proteinPADI5 proteinPDI5 protein |
Information sourced from Uniprot.org