Human P2RX7 protein (Recombinant) (N-His) (STJP005893)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human P2RX7-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | P2RX7 |
| Gene ID | 5027 |
| Uniprot ID | P2RX7_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Asp356-Tyr595 |
Additional Info
| Post Translational Modifications | Phosphorylation results in its inactivation. ADP-ribosylation at Arg-125 is necessary and sufficient to activate P2RX7 and gate the channel. Palmitoylation of several cysteines in the C-terminal cytoplasmic tail is required for efficient localization to cell surface. Palmitoylation prevents channel desensitization by physically anchoring the palmitoylated groups to the membrane. |
| Function | ATP-gated nonselective transmembrane cation channel that requires high millimolar concentrations of ATP for activation. Upon ATP binding, it rapidly opens to allow the influx of small cations Na(+) and Ca(2+), and the K(+) efflux. Also has the ability to form a large pore in the cell membrane, allowing the passage of large cationic molecules. In microglia, may mediate NADPH transport across the plasma membrane. In immune cells, P2RX7 acts as a molecular sensor in pathological inflammatory states by detecting and responding to high local concentrations of extracellar ATP. In microglial cells, P2RX7 activation leads to the release of pro-inflammatory cytokines, such as IL-1beta and IL-18, through the activation of the NLRP3 inflammasome and caspase-1. Cooperates with KCNK6 to activate NLRP3 inflammasome. Activates death pathways leading to apoptosis and autophagy. Activates death pathways leading to pyroptosis. Isoform B: Shows ion channel activity but no macropore function. Isoform H: Non-functional channel. Isoform J: Non-functional channel. |
| Protein Name | P2x Purinoceptor 7P2x7Atp ReceptorP2z ReceptorPurinergic Receptor |
| Database Links | Reactome: R-HSA-139853Reactome: R-HSA-418346Reactome: R-HSA-844456Reactome: R-HSA-9660826Reactome: R-HSA-9856532 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative Protein Names | P2x Purinoceptor 7 proteinP2x7 proteinAtp Receptor proteinP2z Receptor proteinPurinergic Receptor proteinP2RX7 protein |
Information sourced from Uniprot.org