Human P 1 NP (Collagen Alpha-1 (I Chain) Antibody Pair (STJAP00147)
General Information
| Short Description | This P 1 NP antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Mouse |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A.Detection Purification: Protein A. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | COL1A1 |
| Gene ID | 1277 |
| Uniprot ID | CO1A1_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human P 1 NP protein.Detection Immunogen: Recombinant Human P 1 NP protein. |
Additional Info
| Post Translational Modifications | Contains mostly 4-hydroxyproline. Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Contains 3-hydroxyproline at a few sites. This modification occurs on the first proline residue in the sequence motif Gly-Pro-Hyp, where Hyp is 4-hydroxyproline. Lysine residues at the third position of the tripeptide repeating unit (G-X-Y) are 5-hydroxylated in some or all of the chains. O-glycosylated on hydroxylated lysine residues. The O-linked glycan consists of a Glc-Gal disaccharide. |
| Function | Type I collagen is a member of group I collagen (fibrillar forming collagen). |
| Protein Name | Collagen Alpha-1(I ChainAlpha-1 Type I Collagen |
| Database Links | Reactome: R-HSA-114604Reactome: R-HSA-1442490Reactome: R-HSA-1474244Reactome: R-HSA-1650814Reactome: R-HSA-198933Reactome: R-HSA-2022090Reactome: R-HSA-202733Reactome: R-HSA-216083Reactome: R-HSA-2214320Reactome: R-HSA-2243919Reactome: R-HSA-3000170Reactome: R-HSA-3000171Reactome: R-HSA-3000178Reactome: R-HSA-3000480Reactome: R-HSA-430116Reactome: R-HSA-75892Reactome: R-HSA-76009Reactome: R-HSA-8874081Reactome: R-HSA-8940973Reactome: R-HSA-8948216Reactome: R-HSA-9845619Reactome: R-HSA-9845620Reactome: R-HSA-9845621Reactome: R-HSA-9845622Reactome: R-HSA-9846298 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular Matrix |
| Alternative Antibody Pair Names | Collagen Alpha-1(I Chain antibody pairAlpha-1 Type I Collagen antibody pairCOL1A1 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human P 1 NP (Collagen Alpha-1 (I Chain) Antibody Pair (STJAP00147)
This antibody pair detects Human P1NP, the N-terminal propeptide of Collagen Alpha-1 (I Chain), corresponding to UniProt entry P02452. Reactivity is confirmed for human samples only.
The capture antibody is unconjugated and the detection antibody is biotinylated. Both are mouse monoclonal antibodies supplied at 0.5 mg/mL, purified by Protein A, and formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.4.
This antibody pair has been validated for use in ELISA. No other assay formats are documented for this product.
Both antibodies were raised against recombinant Human P1NP protein. The same immunogen was used for both the capture and detection components.
Both components should be stored at -20 degrees Celsius for long-term storage. Freeze-thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.