Human Osteopontin (Osteopontin) Antibody Pair (STJAP00900)
General Information
| Short Description | This Osteopontin antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R01-2W-1/R04-2M-4 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Purification |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | SPP1 |
| Gene ID | 6696 |
| Uniprot ID | OSTP_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | Detected in cerebrospinal fluid and urine (at protein level). Bone. Found in plasma. |
| Post Translational Modifications | Forms covalent cross-links mediated by transglutaminase TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers, increasing its collagen binding properties. Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif. The phosphorylated form inhibits hydroxyapatite crystallization. Dephosphorylation via a mechanism involving ALPL/TNAP promotes hydroxyapatite crystallization. O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62. |
| Function | Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. |
| Protein Name | OsteopontinBone Sialoprotein 1NephropontinSecreted Phosphoprotein 1Spp-1Urinary Stone ProteinUropontin |
| Database Links | Reactome: R-HSA-1474228Reactome: R-HSA-186797Reactome: R-HSA-216083Reactome: R-HSA-381426Reactome: R-HSA-8949275Reactome: R-HSA-8957275Reactome: R-HSA-9856532 |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | Osteopontin antibody pairBone Sialoprotein 1 antibody pairNephropontin antibody pairSecreted Phosphoprotein 1 antibody pairSpp-1 antibody pairUrinary Stone Protein antibody pairUropontin antibody pairSPP1 antibody pairBNSP antibody pairOPN antibody pairPSEC0156 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human Osteopontin (Osteopontin) Antibody Pair (STJAP00900)
This antibody pair detects human Osteopontin (UniProt P10451) and has confirmed reactivity with human samples only.
Both antibodies are rabbit monoclonal IgGs, with clone IDs R01-2W-1 and R04-2M-4. Both were raised against a recombinant protein immunogen and purified by affinity purification.
This antibody pair is validated for use in ELISA. It is supplied unconjugated, so the researcher is responsible for assigning capture and detection roles and optimising antibody concentrations for their specific assay setup.
Both antibodies are formulated in 0.01M PBS at pH 7.2 and are supplied unconjugated.
The antibodies should be stored at 4°C for short-term use. For long-term storage, aliquot and store at -20°C. Repeated freeze/thaw cycles should be avoided to maintain stability.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.