Human Osteonectin/SPARC protein (Recombinant) (C-His) (STJP000988)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human Osteonectin/SPARC Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala18-Ile303) of human Osteonectin/SPARC (Accession #NP_003109.1) fused with a 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human Osteonectin/SPARC-C-His protein was developed in hek293 cells using the region Ala18-Ile303. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | SPARC |
| Gene ID | 6678 |
| Uniprot ID | SPRC_HUMAN |
| Immunogen | Recombinant Human Osteonectin/SPARC Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala18-Ile303) of human Osteonectin/SPARC (Accession #NP_003109.1) fused with a 6×His tag at the C-terminus. |
| Immunogen Region | Ala18-Ile303 |
| Immunogen Sequence | APQQEALPDETEVVEETVAE VTEVSVGANPVQVEVGEFDD GAEETEEEVVAENPCQNHHC KHGKVCELDENNTPMCVCQD PTSCPAPIGEFEKVCSNDNK TFDSSCHFFATKCTLEGTKK GHKLHLDYIGPCKYIPPCLD SELTEFPLRMRDWLKNVLVT LYERDEDNNLLTEKQKLRVK KIHENEKRLEAGDHPVELLA RDFEKNYNMYIFPVHWQFGQ LDQHPIDGYLSHTELAPLR |
Additional Info
| Function | Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites.an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. |
| Protein Name | SparcBasement-Membrane Protein 40Bm-40OsteonectinOnSecreted Protein Acidic And Rich In Cysteine |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-1251985Reactome: R-HSA-3000178Reactome: R-HSA-3000497 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular MatrixBasement MembraneIn Or Around The Basement Membrane |
| Alternative Protein Names | Sparc proteinBasement-Membrane Protein 40 proteinBm-40 proteinOsteonectin proteinOn proteinSecreted Protein Acidic And Rich In Cysteine proteinSPARC proteinON protein |
Information sourced from Uniprot.org