Human ORM2 (Alpha-1-acid glycoprotein 2) CLIA Kit for Quantitative Detection

SPECIFICATIONS
STJC0000749
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General Information

Short DescriptionThis human ORM2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
ApplicationsCLIA
ReactivityHuman
Sensitivity9.38pg/mL
Detection Limit15.63~1000pg/mL
NoteFOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage InstructionIf unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time3.5h
DetectionChemiluminescence

Target Information

Gene SymbolORM2
Gene ID5005
Uniprot IDA1AG2_HUMAN
SpecificityThis kit recognizes Human ORM2 in samples. No significant cross-reactivity or interference between Human ORM2 and analogues was observed.
Sample TypeSerum, plasma and other biological fluids

Additional Info

Tissue Specificity Expressed by the liver and secreted in plasma.
Post Translational Modifications N-glycosylated. N-glycan heterogeneity at Asn-33: Hex5HexNAc4 (minor), Hex6HexNAc5 (major) and dHex1Hex6HexNAc5 (minor).
Function Functions as a transport protein in the blood stream. Binds various hydrophobic ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability. Appears to function in modulating the activity of the immune system during the acute-phase reaction.
Protein Name Alpha-1-Acid Glycoprotein 2
Agp 2
Orosomucoid-2
Omd 2
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-6798695
Cellular Localisation Secreted
Alternative CLIA Names Alpha-1-Acid Glycoprotein 2 CLIA kit
Agp 2 CLIA kit
Orosomucoid-2 CLIA kit
Omd 2 CLIA kit
ORM2 CLIA kit
AGP2 CLIA kit
Specificity This kit recognizes Human ORM2 in samples. No significant cross-reactivity or interference between Human ORM2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 96-110 102
EDTA plasma(n=8) 97-115 105
Cell culture media(n=8) 86-101 93

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 48.03 111.45 464.81 48.00 102.91 478.42
Standard deviation 5.86 9.27 43.92 4.32 10.25 53.87
CV (%) 12.20 8.32 9.45 9.00 9.96 11.26

Citations

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