Human ON (Sparc) Antibody Pair (STJAP00229)
General Information
| Short Description | This ON antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Rabbit |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Polyclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A.Detection Purification: Protein A. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | SPARC |
| Gene ID | 6678 |
| Uniprot ID | SPRC_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human ON protein.Detection Immunogen: Recombinant Human ON protein. |
Additional Info
| Protein Name | SparcBasement-Membrane Protein 40Bm-40OsteonectinOnSecreted Protein Acidic And Rich In Cysteine |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-1251985Reactome: R-HSA-3000178Reactome: R-HSA-3000497 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular MatrixBasement MembraneIn Or Around The Basement Membrane |
| Alternative Antibody Pair Names | Sparc antibody pairBasement-Membrane Protein 40 antibody pairBm-40 antibody pairOsteonectin antibody pairOn antibody pairSecreted Protein Acidic And Rich In Cysteine antibody pairSPARC antibody pairON antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human ON (Sparc) Antibody Pair (STJAP00229)
This antibody pair detects human ON (SPARC, UniProt P09486) and is validated for reactivity with human samples only. Both the capture and detection antibodies were raised against recombinant human ON protein.
The capture antibody is an unconjugated mouse monoclonal antibody and the detection antibody is a biotin-conjugated rabbit polyclonal antibody. Both are supplied at 0.5 mg/mL, formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.4.
This antibody pair has been validated for use in ELISA. No other assay formats are listed in the product documentation.
Both the capture and detection antibodies were purified using Protein A affinity chromatography.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain reagent integrity.
No. This product is intended for in vitro research use only and is not approved or validated for diagnostic, therapeutic, or clinical applications.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.