Human NT5E (5 NA-nucleotidase) CLIA Kit for Quantitative Detection

Name: Human NT5E (5 NA-nucleotidase) CLIA Kit for Quantitative Detection
SKU: STJC0000159
Availability: InStock

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SPECIFICATIONS

STJC0000159

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Short Description	This human NT5E kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
Applications	CLIA
Reactivity	Human
Sensitivity	37.5pg/mL
Detection Limit	62.5~4000pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Chemiluminescence

Gene Symbol	NT5E
Gene ID	4907
Uniprot ID	5NTD_HUMAN
Specificity	This kit recognizes Human 5-NT in samples. No significant cross-reactivity or interference between Human 5-NT and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Function	Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate. Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms. Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN.
Protein Name	5'-Nucleotidase 5'-Nt 5'-Deoxynucleotidase Ecto-5'-Nucleotidase Imp-Specific 5'-Nucleotidase Thymidylate 5'-Phosphatase Cd Antigen Cd73
Database Links	Reactome: R-HSA-196807 Reactome: R-HSA-73621 Reactome: R-HSA-74259 Reactome: R-HSA-9660826
Cellular Localisation	Cell Membrane Lipid-Anchor Gpi-Anchor
Alternative CLIA Names	5'-Nucleotidase CLIA kit 5'-Nt CLIA kit 5'-Deoxynucleotidase CLIA kit Ecto-5'-Nucleotidase CLIA kit Imp-Specific 5'-Nucleotidase CLIA kit Thymidylate 5'-Phosphatase CLIA kit Cd Antigen Cd73 CLIA kit NT5E CLIA kit NT5 CLIA kit NTE CLIA kit
Specificity	This kit recognizes Human 5-NT in samples. No significant cross-reactivity or interference between Human 5-NT and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	199.21	595.70	1640.79	210.42	621.13	1523.54
Standard deviation	16.26	48.55	179.99	18.29	52.73	155.40
CV (%)	8.16	8.15	10.97	8.69	8.49	10.20