This NOX5 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Applications
ELISA
Reactivity
Human
Sensitivity
18.75pg/mL
Detection Limit
31.25~2000pg/mL
Note
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Product Properties
Storage Instruction
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
This kit recognizes Human NOX5 in samples. No significant cross-reactivity or interference between Human NOX5 and analogues was observed.
Sample Type
Serum, plasma and other biological fluids
Additional Info
Post Translational Modifications
Isoform v2: Phosphorylation at Ser-475 by CaMK2 and at Ser-490, Thr-494 and Ser-498 by PKC/PRKCA positively regulates its catalytic activity. Isoform v2: S-nitrosylation in response to nitric oxide inhibits its catalytic activity.
Function
Calcium-dependent NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor. May play a role in cell growth and apoptosis. Isoform v2: Calcium-dependent NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor. Involved in endothelial generation of reactive oxygen species (ROS), proliferation and angiogenesis and contributes to endothelial response to thrombin. Regulates redox-dependent processes in lymphocytes and spermatozoa. Isoform v1: Calcium-dependent NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor. Isoform v5: This isoform lacks calcium-binding domains and was showed to present a NADPH oxidase activity in a calcium-independent manner. May be involved in endothelial generation of reactive oxygen species (ROS), proliferation and angiogenesis and contribute to endothelial response to thrombin. However another study showed an absence of oxidase activity. Subject to rapid degradation. Isoform v3: Lacks calcium-dependent NADPH oxidase activity. Isoform v4: Lacks calcium-dependent NADPH oxidase activity.
Isoform V2: Endoplasmic ReticulumCell MembraneMulti-Pass Membrane ProteinCalcium-Sensitive Association And Dissociation Between The N- And C-Terminal Domains Appears To Facilitate Its Localization To The Cell MembraneIsoform V5: Endoplasmic Reticulum
Alternative ELISA Names
Nadph Oxidase 5 ELISA kitNOX5 ELISA kit
Specificity
This kit recognizes Human NOX5 in samples. No significant cross-reactivity or interference between Human NOX5 and analogues was observed.
