Human Notch 3 protein (Recombinant) (C-His&Avi) (STJP020311)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human Notch 3 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.；It contains Ala40-Glu467.
General Information
| Short Description | Recombinant-Human Notch 3-C-His&Avi protein was developed in hek293 cells using the region Ala40-Glu467. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | < 1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | NOTCH3 |
| Gene ID | 4854 |
| Uniprot ID | NOTC3_HUMAN |
| Immunogen | Recombinant Human Notch 3 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.；It contains Ala40-Glu467. |
| Immunogen Region | Ala40-Glu467 |
Additional Info
| Tissue Specificity | Ubiquitously expressed in fetal and adult tissues. |
| Post Translational Modifications | Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane. Phosphorylated. Hydroxylated by HIF1AN. |
| Function | Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. |
| Protein Name | Neurogenic Locus Notch Homolog Protein 3Notch 3 Cleaved Into - Notch 3 Extracellular Truncation - Notch 3 Intracellular Domain |
| Database Links | Reactome: R-HSA-1912399Reactome: R-HSA-1912408Reactome: R-HSA-1912420Reactome: R-HSA-350054Reactome: R-HSA-5083630Reactome: R-HSA-9013507Reactome: R-HSA-9013508Reactome: R-HSA-9017802 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinNotch 3 Intracellular Domain: NucleusFollowing Proteolytical Processing Nicd Is Translocated To The Nucleus |
| Alternative Protein Names | Neurogenic Locus Notch Homolog Protein 3 proteinNotch 3 Cleaved Into - Notch 3 Extracellular Truncation - Notch 3 Intracellular Domain proteinNOTCH3 protein |
Information sourced from Uniprot.org