• Biotinylated Human Notch 3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
  • The purity of Biotinylated Human Notch 3 is greater than 95% as determined by SEC-HPLC.

Human Notch 3 protein (Recombinant) (C-His-Avi) {Biotin} (STJP001557)

SKU:
STJP001557

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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human Notch 3-C-His-Avi protein was developed from hek293 and has a target region of C-His-Avi. For use in research applications.
Conjugation: Biotin
Formulation: Lyophilised from 0.2 µm filtered PBS solution pH7.4.
Dilution Range: Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM
Storage Instruction: The lyophilized protein is stable for at least 1 year from date of receipt at-20°C.
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg).
Gene Symbol: NOTCH3
Gene ID: 4854
Uniprot ID: NOTC3_HUMAN
Immunogen Region: Ala40-Glu467
Immunogen: DNA sequence encoding Human Notch 3 including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Post Translational Modifications Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane. Phosphorylated. Hydroxylated by HIF1AN.
Function Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.
Protein Name Neurogenic Locus Notch Homolog Protein 3
Notch 3 Cleaved Into - Notch 3 Extracellular Truncation - Notch 3 Intracellular Domain
Database Links Reactome: R-HSA-1912399
Reactome: R-HSA-1912408
Reactome: R-HSA-1912420
Reactome: R-HSA-350054
Reactome: R-HSA-5083630
Reactome: R-HSA-9013507
Reactome: R-HSA-9013508
Reactome: R-HSA-9017802
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Notch 3 Intracellular Domain: Nucleus
Following Proteolytical Processing Nicd Is Translocated To The Nucleus
Alternative Protein Names Neurogenic Locus Notch Homolog Protein 3 protein
Notch 3 Cleaved Into - Notch 3 Extracellular Truncation - Notch 3 Intracellular Domain protein
NOTCH3 protein

Information sourced from Uniprot.org

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