Human NOS3 (Nitric oxide synthase, endothelial) Sandwich ELISA Kit (STJE0002459)

Name: Human NOS3 (Nitric oxide synthase, endothelial) Sandwich ELISA Kit (STJE0002459)
SKU: STJE0002459
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

STJE0002459

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General Information

Short Description	This NOS3 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Human
Sensitivity	37.5pg/mL
Detection Limit	62.5~4000pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Target Information

Gene Symbol	NOS3
Gene ID	4846
Uniprot ID	NOS3_HUMAN
Specificity	This kit recognizes Human NOS3/eNOS in samples. No significant cross-reactivity or interference between Human NOS3/eNOS and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Additional Info

Post Translational Modifications	Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity. Phosphorylation of Ser-114 by CDK5 reduces activity.
Function	Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
Protein Name	Nitric Oxide Synthase 3 Constitutive Nos Cnos Ec-Nos Nos Type Iii Nosiii Nitric Oxide Synthase - Endothelial Endothelial Nos Enos
Database Links	Reactome: R-HSA-1222556 Reactome: R-HSA-1474151 Reactome: R-HSA-203615 Reactome: R-HSA-203641 Reactome: R-HSA-203754 Reactome: R-HSA-392154 Reactome: R-HSA-5218920 Reactome: R-HSA-9009391 Reactome: R-HSA-9856530
Cellular Localisation	Cell Membrane Membrane Caveola Cytoplasm Cytoskeleton Golgi Apparatus Specifically Associates With Actin Cytoskeleton In The G2 Phase Of The Cell Cycle Which Is Favored By Interaction With Nosip And Results In A Reduced Enzymatic Activity
Alternative ELISA Names	Nitric Oxide Synthase 3 ELISA kit Constitutive Nos ELISA kit Cnos ELISA kit Ec-Nos ELISA kit Nos Type Iii ELISA kit Nosiii ELISA kit Nitric Oxide Synthase - Endothelial ELISA kit Endothelial Nos ELISA kit Enos ELISA kit NOS3 ELISA kit
Specificity	This kit recognizes Human NOS3/eNOS in samples. No significant cross-reactivity or interference between Human NOS3/eNOS and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	93-108	101
EDTA plasma (n=8)	94-106	99
Cell culture media (n=8)	92-104	98

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	186.70	451.70	1380.60	204.80	422.50	1440.90
Standard deviation	12.90	20.30	69.00	12.70	18.60	46.10
CV (%)	6.91	4.49	5.00	6.20	4.40	3.20

Citations

View product citations for antibody STJE0002459 on CiteAb