Human NOS2 (Nitric oxide synthase, inducible) Sandwich ELISA Kit (STJE0002457)

Name: Human NOS2 (Nitric oxide synthase, inducible) Sandwich ELISA Kit (STJE0002457)
SKU: STJE0002457
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

STJE0002457

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General Information

Short Description	This NOS2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Human
Sensitivity	46.88pg/mL
Detection Limit	78.13~5000pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Target Information

Gene Symbol	NOS2
Gene ID	4843
Uniprot ID	NOS2_HUMAN
Specificity	This kit recognizes Human NOS2/iNOS in samples. No significant cross-reactivity or interference between Human NOS2/iNOS and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Additional Info

Post Translational Modifications	Polyubiquitinated.mediated by SPSB1, SPSB2 and SPSB4, leading to proteasomal degradation.
Function	Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8.
Protein Name	Nitric Oxide Synthase - Inducible Hepatocyte Nos Hep-Nos Inducible No Synthase Inducible Nos Inos Nos Type Ii Peptidyl-Cysteine S-Nitrosylase Nos2
Database Links	Reactome: R-HSA-1222556 Reactome: R-HSA-392154 Reactome: R-HSA-6785807 Reactome: R-HSA-9033241 Reactome: R-HSA-9636249
Cellular Localisation	Cytoplasm Cytosol Localizes As Discrete Foci Scattered Throughout The Cytosol And In The Presence Of Spsb1 And Spsb4 Exhibits A More Diffuse Cytosolic Localization
Alternative ELISA Names	Nitric Oxide Synthase - Inducible ELISA kit Hepatocyte Nos ELISA kit Hep-Nos ELISA kit Inducible No Synthase ELISA kit Inducible Nos ELISA kit Inos ELISA kit Nos Type Ii ELISA kit Peptidyl-Cysteine S-Nitrosylase Nos2 ELISA kit NOS2 ELISA kit NOS2A ELISA kit
Specificity	This kit recognizes Human NOS2/iNOS in samples. No significant cross-reactivity or interference between Human NOS2/iNOS and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	95-108	101
EDTA plasma (n=8)	94-107	99
Cell culture media (n=8)	85-95	90

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	276.20	441.40	2054.00	265.80	481.50	2256.90
Standard deviation	14.10	24.70	71.90	17.30	26.50	92.50
CV (%)	5.10	5.60	3.50	6.51	5.50	4.10

Citations

View product citations for antibody STJE0002457 on CiteAb