Human NOS1 (Nitric oxide synthase, brain) Sandwich ELISA Kit (STJE0002447)

Name: Human NOS1 (Nitric oxide synthase, brain) Sandwich ELISA Kit (STJE0002447)
SKU: STJE0002447
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

STJE0002447

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Short Description	This NOS1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Human
Sensitivity	0.09ng/mL
Detection Limit	0.16~10ng/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Gene Symbol	NOS1
Gene ID	4842
Uniprot ID	NOS1_HUMAN
Specificity	This kit recognizes Human NOS1/nNOS in samples. No significant cross-reactivity or interference between Human NOS1/nNOS and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Post Translational Modifications	Ubiquitinated.mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).
Function	Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
Protein Name	Nitric Oxide Synthase 1 Constitutive Nos Nc-Nos Nos Type I Neuronal Nos N-Nos Nnos Nitric Oxide Synthase - Brain Bnos Peptidyl-Cysteine S-Nitrosylase Nos1
Database Links	Reactome: R-HSA-1222556 Reactome: R-HSA-392154 Reactome: R-HSA-5578775
Cellular Localisation	Cell Membrane Sarcolemma Peripheral Membrane Protein Cell Projection Dendritic Spine In Skeletal Muscle It Is Localized Beneath The Sarcolemma Of Fast-Twitch Muscle Fiber By Associating With The Dystrophin Glycoprotein Complex In Neurons Enriched In Dendritic Spines
Alternative ELISA Names	Nitric Oxide Synthase 1 ELISA kit Constitutive Nos ELISA kit Nc-Nos ELISA kit Nos Type I ELISA kit Neuronal Nos ELISA kit N-Nos ELISA kit Nnos ELISA kit Nitric Oxide Synthase - Brain ELISA kit Bnos ELISA kit Peptidyl-Cysteine S-Nitrosylase Nos1 ELISA kit NOS1 ELISA kit
Specificity	This kit recognizes Human NOS1/nNOS in samples. No significant cross-reactivity or interference between Human NOS1/nNOS and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	0.50	1.40	3.50	0.50	1.30	3.40
Standard deviation	0.03	0.07	0.19	0.03	0.05	0.16
CV (%)	6.00	5.00	5.43	6.00	3.85	4.71