Human NMP22 (Nuclear Mitotic Apparatus Protein 1) Antibody Pair (STJAP00140)
SPECIFICATIONS
ClonalityMonoclonal/Monoclonal
HostMouse/Mouse
ConjugationUnconjugated/Biotin
ImmunogenCapture immunogen: Recombinant Human NMP22 protein.Detection Immunogen: Recombinant Human NMP22 protein.
General Information
| Short Description | This NMP22 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Mouse |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A.Detection Purification: Protein A. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | NUMA1 |
| Gene ID | 4926 |
| Uniprot ID | NUMA1_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human NMP22 protein.Detection Immunogen: Recombinant Human NMP22 protein. |
Additional Info
| Function | Microtubule (MT)-binding protein that plays a role in the formation and maintenance of the spindle poles and the alignement and the segregation of chromosomes during mitotic cell division. Functions to tether the minus ends of MTs at the spindle poles, which is critical for the establishment and maintenance of the spindle poles. Plays a role in the establishment of the mitotic spindle orientation during metaphase and elongation during anaphase in a dynein-dynactin-dependent manner. In metaphase, part of a ternary complex composed of GPSM2 and G(i) alpha proteins, that regulates the recruitment and anchorage of the dynein-dynactin complex in the mitotic cell cortex regions situated above the two spindle poles, and hence regulates the correct oritentation of the mitotic spindle. During anaphase, mediates the recruitment and accumulation of the dynein-dynactin complex at the cell membrane of the polar cortical region through direct association with phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), and hence participates in the regulation of the spindle elongation and chromosome segregation. Also binds to other polyanionic phosphoinositides, such as phosphatidylinositol 3-phosphate (PIP), lysophosphatidic acid (LPA) and phosphatidylinositol triphosphate (PIP3), in vitro. Also required for proper orientation of the mitotic spindle during asymmetric cell divisions. Plays a role in mitotic MT aster assembly. Involved in anastral spindle assembly. Positively regulates TNKS protein localization to spindle poles in mitosis. Highly abundant component of the nuclear matrix where it may serve a non-mitotic structural role, occupies the majority of the nuclear volume. Required for epidermal differentiation and hair follicle morphogenesis. |
| Protein Name | Nuclear Mitotic Apparatus Protein 1Nuclear Matrix Protein-22Nmp-22Nuclear Mitotic Apparatus ProteinNuma ProteinSp-H Antigen |
| Database Links | Reactome: R-HSA-380320Reactome: R-HSA-68875 |
| Cellular Localisation | NucleusNucleoplasmNucleus MatrixChromosomeCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeSpindle PoleCell CortexCell MembraneLipid-AnchorCytoplasmic SideLateral Cell MembraneMitotic Cell Cycle-Dependent Shuttling Protein That Relocalizes From The Interphase Nucleus To The Spindle Poles And Cell CortexThe Localization To The Spindle Poles Is Regulated By AaasIn InterphaseResides In The Nuclear MatrixIn ProphaseRestricted To The Interchromatin Or Condensed Chromosome SpaceIn PrometaphaseAfter Nuclear Envelope DisassemblyForms Aggregates Both In The Spindle Midzone And At Duplicated Centrosomes And Astral Microtubules (Mts) Of The Bipolar Spindle ApparatusTranslocates From The Spindle Midzone Towards The Spindle Poles Along Spindle Fibers In A Mt- And Dynein-Dynactin-Dependent Manner Until The Anaphase OnsetIn MetaphaseRecruited To The Polar Cortical Region In A Gpsm2- And Gnai1-Dependent MannerExcluded From The Metaphase Equatorial Cortical Region In A Rangtp-Dependent MannerPhosphorylation On Thr-2055 By Cdk1 Results In Its Localization At Spindle Poles In MetaphaseBut Not At The Cell CortexIn AnaphaseRecruited And Anchored At The Cell Membrane Of The Polar Cortical Region In A Epb41-Epb41l2-Phosphatidylinositol-Dependent And Gpsm2- And G(I) Alpha Proteins-Independent MannerExcluded From The Anaphase Equatorial Region Of The Cell Cortex In A Racgap1- And Kif23-Dependent And Rangtp-Independent MannerAssociated With Astral Mts Emanating From The Spindle Poles During AnaphaseNonphosphorylated Thr-2055 Localizes At The Cell CortexWeakly During Metaphase And More Prominently During Anaphase In A Phosphatase Ppp2ca-Dependent MannerAs Mitosis Progresses It Reassociates With Telophase Chromosomes Very Early During Nuclear ReformationBefore Substantial Accumulation Of Lamins On Chromosomal Surfaces Is EvidentLocalizes To The Tips Of Cortical Mts In PrometaphaseLocalizes Along Mts And Specifically To Both Mt Plus And Minus EndsAlso Accumulates At Mt Tips Near The Cell PeripheryColocalizes With Gpsm2 At Mitotic Spindle Poles During MitosisColocalizes With Spag5 At Mitotic Spindle At Prometaphase And At Mitotic Spindle Poles At Metaphase And AnaphaseColocalizes With Abro1 At Mitotic Spindle PolesColocalized With Tnks From Prophase Through To Anaphase In MitosisColocalizes With Tubulin AlphaCcsap Is Essential For Its Centrosomal LocalizationIn Horizontally Retinal Progenitor Dividing CellsLocalized To The Lateral Cortical RegionIsoform 3: CytoplasmCytosolDuring InterphaseMainly Clustered At The Centrosomal Region In The CytosolAfter Entry Into MitosisDetected At Mitotic Spindle PolesIsoform 4: Cytoplasm |
| Alternative Antibody Pair Names | Nuclear Mitotic Apparatus Protein 1 antibody pairNuclear Matrix Protein-22 antibody pairNmp-22 antibody pairNuclear Mitotic Apparatus Protein antibody pairNuma Protein antibody pairSp-H Antigen antibody pairNUMA1 antibody pairNMP22 antibody pairNUMA antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human NMP22 (Nuclear Mitotic Apparatus Protein 1) Antibody Pair (STJAP00140)
This antibody pair detects human Nuclear Mitotic Apparatus Protein 1 (NMP22), corresponding to UniProt entry Q14980. Reactivity is confirmed for human samples only.
The capture antibody is unconjugated and the detection antibody is biotinylated. Both are mouse monoclonal antibodies supplied at 0.5 mg/mL, formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.4.
This pair is validated for use in ELISA. No other assay formats are listed in the product specifications.
Both the capture and detection antibodies were raised against recombinant human NMP22 protein. No additional immunogen region information is specified.
Both antibodies were purified by Protein A affinity chromatography.
Both components should be stored at -20°C for long-term storage. Repeated freeze-thaw cycles should be avoided to maintain reagent integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.