Human NME4 protein (Recombinant) (N-His) (STJP003171)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human NME4-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | NME4 |
| Gene ID | 4833 |
| Uniprot ID | NDKM_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Thr67-His185 |
Additional Info
| Function | Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Through the catalyzed exchange of gamma-phosphate between di- and triphosphonucleosides participates in regulation of intracellular nucleotide homeostasis. Binds to anionic phospholipids, predominantly to cardiolipin.the binding inhibits its phosphotransfer activity. Acts as a mitochondria-specific NDK.its association with cardiolipin-containing mitochondrial inner membrane is coupled to respiration suggesting that ADP locally regenerated in the mitochondrion innermembrane space by its activity is directly taken up via ANT ADP/ATP translocase into the matrix space to stimulate respiratory ATP regeneration. Proposed to increase GTP-loading on dynamin-related GTPase OPA1 in mitochondria. In vitro can induce liposome cross-linking suggesting that it can cross-link inner and outer membranes to form contact sites, and promotes intermembrane migration of anionic phosphoplipids. Promotes the redistribution of cardiolipin between the mitochondrial inner membrane and outer membrane which is implicated in pro-apoptotic signaling. |
| Protein Name | Nucleoside Diphosphate Kinase - MitochondrialNdkNdp Kinase - MitochondrialNucleoside Diphosphate Kinase DNdpkdNm23-H4 |
| Database Links | Reactome: R-HSA-499943 |
| Cellular Localisation | Mitochondrion Intermembrane SpacePeripheral Membrane ProteinMitochondrion MatrixPredominantly Localized In The Mitochondrion Intermembrane SpaceColocalizes With Opa1 In Mitochondria |
| Alternative Protein Names | Nucleoside Diphosphate Kinase - Mitochondrial proteinNdk proteinNdp Kinase - Mitochondrial proteinNucleoside Diphosphate Kinase D proteinNdpkd proteinNm23-H4 proteinNME4 proteinNM23D protein |
Information sourced from Uniprot.org