• Biotinylated Human NKG2A&CD94 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
  • The purity of Biotinylated Human NKG2A&CD94 is greater than 95% as determined by SEC-HPLC.
  • Immobilized Anti-NKG2A Antibody, hFc Tag at 1 Mu g/ml (100 Mu l/Well) on the plate. Dose response curve for Biotinylated Human NKG2A&CD94, His Tag with the EC50 of 0. 11 Mu g/ml determined by ELISA.

Human NKG2A&CD94 protein (Recombinant) (N-His-Avi) {Biotin} (STJP001591)

SKU:
STJP001591

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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human NKG2A&CD94-N-His-Avi protein was developed from hek293 and has a target region of N-His-Avi. For use in research applications.
Conjugation: Biotin
Formulation: Lyophilised from 0.2 µm filtered PBS solution pH7.4.
Dilution Range: Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM
Storage Instruction: The lyophilized protein is stable for at least 1 year from date of receipt at-20°C.
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg).
Gene Symbol: KLRC1
Gene ID: 3821
Uniprot ID: NKG2A_HUMAN
Immunogen Region: Arg100-Leu233 (NKG2A) & Ser34-Ile179 (CD94)
Immunogen: DNA sequence encoding Human NKG2A&CD94 including a N-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Post Translational Modifications Phosphorylated.
Function Immune inhibitory receptor involved in self-nonself discrimination. In complex with KLRD1 on cytotoxic and regulatory lymphocyte subsets, recognizes non-classical major histocompatibility (MHC) class Ib molecule HLA-E loaded with self-peptides derived from the signal sequence of classical MHC class Ia molecules. Enables cytotoxic cells to monitor the expression of MHC class I molecules in healthy cells and to tolerate self. Upon HLA-E-peptide binding, transmits intracellular signals through two immunoreceptor tyrosine-based inhibition motifs (ITIMs) by recruiting INPP5D/SHP-1 and INPPL1/SHP-2 tyrosine phosphatases to ITIMs, and ultimately opposing signals transmitted by activating receptors through dephosphorylation of proximal signaling molecules. Key inhibitory receptor on natural killer (NK) cells that regulates their activation and effector functions. Dominantly counteracts T cell receptor signaling on a subset of memory/effector CD8-positive T cells as part of an antigen-driven response to avoid autoimmunity. On intraepithelial CD8-positive gamma-delta regulatory T cells triggers TGFB1 secretion, which in turn limits the cytotoxic programming of intraepithelial CD8-positive alpha-beta T cells, distinguishing harmless from pathogenic antigens. In HLA-E-rich tumor microenvironment, acts as an immune inhibitory checkpoint and may contribute to progressive loss of effector functions of NK cells and tumor-specific T cells, a state known as cell exhaustion. (Microbial infection) Viruses like human cytomegalovirus have evolved an escape mechanism whereby virus-induced down-regulation of host MHC class I molecules is coupled to the binding of viral peptides to HLA-E, restoring HLA-E expression and inducing HLA-E-dependent NK cell immune tolerance to infected cells. Recognizes HLA-E in complex with human cytomegalovirus UL40-derived peptide (VMAPRTLIL) and inhibits NK cell cytotoxicity. (Microbial infection) May recognize HLA-E in complex with HIV-1 gag/Capsid protein p24-derived peptide (AISPRTLNA) on infected cells and may inhibit NK cell cytotoxicity, a mechanism that allows HIV-1 to escape immune recognition. (Microbial infection) Upon SARS-CoV-2 infection, may contribute to functional exhaustion of cytotoxic NK cells and CD8-positive T cells. On NK cells, may recognize HLA-E in complex with SARS-CoV-2 S/Spike protein S1-derived peptide (LQPRTFLL) expressed on the surface of lung epithelial cells, inducing NK cell exhaustion and dampening antiviral immune surveillance.
Protein Name Nkg2-A/Nkg2-B Type Ii Integral Membrane Protein
Cd159 Antigen-Like Family Member A
Nk Cell Receptor A
Nkg2-A/B-Activating Nk Receptor
Cd Antigen Cd159a
Database Links Reactome: R-HSA-198933
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Alternative Protein Names Nkg2-A/Nkg2-B Type Ii Integral Membrane Protein protein
Cd159 Antigen-Like Family Member A protein
Nk Cell Receptor A protein
Nkg2-A/B-Activating Nk Receptor protein
Cd Antigen Cd159a protein
KLRC1 protein
NKG2A protein

Information sourced from Uniprot.org

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