Human NGAL (Neutrophil Gelatinase-Associated Lipocalin) Antibody Pair (STJAP00052)
General Information
| Short Description | This NGAL antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Mouse |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A.Detection Purification: Protein A. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | LCN2 |
| Gene ID | 3934 |
| Uniprot ID | NGAL_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human NGAL protein.Detection Immunogen: Recombinant Human NGAL protein. |
Additional Info
| Function | Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,3-dihydroxybenzoic acid (2,3-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity.limits bacterial proliferation by sequestering iron bound to microbial siderophores, such as enterobactin. Can also bind siderophores from M.tuberculosis. |
| Protein Name | Neutrophil Gelatinase-Associated LipocalinNgal25 Kda Alpha-2-Microglobulin-Related Subunit Of Mmp-9Lipocalin-2Oncogene 24p3SiderocalinP25 |
| Database Links | Reactome: R-HSA-6785807Reactome: R-HSA-6798695Reactome: R-HSA-6799990Reactome: R-HSA-917937 |
| Cellular Localisation | SecretedCytoplasmic Granule LumenCytoplasmic Vesicle LumenUpon Binding To The Slc22a17 (24p3r) ReceptorIt Is InternalizedReleases The Bound Iron In The Acidic Lumen Of Cytoplasmic Vesicles |
| Alternative Antibody Pair Names | Neutrophil Gelatinase-Associated Lipocalin antibody pairNgal antibody pair25 Kda Alpha-2-Microglobulin-Related Subunit Of Mmp-9 antibody pairLipocalin-2 antibody pairOncogene 24p3 antibody pairSiderocalin antibody pairP25 antibody pairLCN2 antibody pairHNL antibody pairNGAL antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human NGAL (Neutrophil Gelatinase-Associated Lipocalin) Antibody Pair (STJAP00052)
This antibody pair detects human Neutrophil Gelatinase-Associated Lipocalin (NGAL), corresponding to UniProt accession P80188. Reactivity has been confirmed for human samples only.
The capture antibody is supplied unconjugated and the detection antibody is biotin-conjugated. Both are mouse monoclonal antibodies purified by Protein A, supplied at 0.5 mg/mL in PBS with 0.04% Proclin 300 and 50% glycerol, pH 7.4.
This antibody pair has been validated for use in ELISA. No other assay formats are listed in the product specifications.
Both the capture and detection antibodies were raised against recombinant human NGAL protein. No immunogen region mapping data is provided in the current product specifications.
Both components should be stored at -20 degrees Celsius for long-term storage. Freeze-thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.