Human NG2 (NG2) Antibody Pair (STJAP00802)
General Information
| Short Description | This NG2 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R52-6C-8/R35-5A-8 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | CSPG4 |
| Gene ID | 1464 |
| Uniprot ID | CSPG4_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | Detected in fibroblasts (at protein level). Detected in placenta (at protein level). Detected in malignant melanoma cells. |
| Post Translational Modifications | O-glycosylated.contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation. Involved in interaction with MMP16 and ITGA4. Phosphorylation by PRKCA regulates its subcellular location and function in cell motility. |
| Function | Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Also functions as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades. |
| Protein Name | Chondroitin Sulfate Proteoglycan 4Chondroitin Sulfate Proteoglycan Ng2Melanoma Chondroitin Sulfate ProteoglycanMelanoma-Associated Chondroitin Sulfate Proteoglycan |
| Database Links | Reactome: R-HSA-1971475Reactome: R-HSA-2022870Reactome: R-HSA-2022923Reactome: R-HSA-2024101Reactome: R-HSA-3560783Reactome: R-HSA-3560801Reactome: R-HSA-3595172Reactome: R-HSA-3595174Reactome: R-HSA-3595177Reactome: R-HSA-4420332Reactome: R-HSA-9725554 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinExtracellular SideApical Cell MembraneCell ProjectionLamellipodium MembraneCell SurfaceLocalized At The Apical Plasma Membrane It Relocalizes To The Lamellipodia Of Astrocytoma Upon Phosphorylation By PrkcaLocalizes To The Retraction FibersLocalizes To The Plasma Membrane Of Oligodendrocytes |
| Alternative Antibody Pair Names | Chondroitin Sulfate Proteoglycan 4 antibody pairChondroitin Sulfate Proteoglycan Ng2 antibody pairMelanoma Chondroitin Sulfate Proteoglycan antibody pairMelanoma-Associated Chondroitin Sulfate Proteoglycan antibody pairCSPG4 antibody pairMCSP antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human NG2 (NG2) Antibody Pair (STJAP00802)
This antibody pair is designed to detect human NG2 (UniProt Q6UVK1) and has confirmed reactivity in human samples only. No cross-reactivity data for other species is provided.
Both the capture and detection antibodies are rabbit monoclonal IgGs supplied unconjugated at 1 mg/mL, formulated in 0.01M PBS at pH 7.2, and purified by affinity chromatography. The clone IDs are R52-6C-8 and R35-5A-8 respectively.
This pair has been validated for use in ELISA. No other assay formats are listed in the product documentation.
Optimal working dilutions for both antibodies are to be determined by the end user. No fixed dilution range is specified in the product details.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquot and keep at -20°C. Repeated freeze/thaw cycles should be avoided to preserve activity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.