Human NF-L/NEFL protein (Recombinant) (No-Tag) (STJP002927)
SPECIFICATIONS
HostPichia
ConjugationUnconjugated
ImmunogenRecombinant Human NF-L/NEFL Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ser60-Thr250) of human NF-L/NEFL (Accession #NP_006149.2) fused with no additional amino acid.
General Information
| Short Description | Recombinant-Human NF-L/NEFL-No-Tag protein was developed in pichia using the region Ser60-Thr250. For use in research applications. |
| Host | Pichia |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | <0.1 EU/Mu g |
Target Information
| Gene Symbol | NEFL |
| Gene ID | 4747 |
| Uniprot ID | NFL_HUMAN |
| Immunogen | Recombinant Human NF-L/NEFL Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ser60-Thr250) of human NF-L/NEFL (Accession #NP_006149.2) fused with no additional amino acid. |
| Immunogen Region | Ser60-Thr250 |
| Immunogen Sequence | INYKQLQLQERTNIRKCQEL LEQLNGKINLTYRADFKIPM EMTEKMQKSYTAFAIQEMLQ NVFLVFRNNFSSTGWNETIV VRLLDELHQQTVFLKTVLEE KQEERLTWEMSSTALHLKSY YWRVQRYLKLMKYNSYAWMV VRAEIFRNFLIIRRLTRNFQ N |
Additional Info
| Post Translational Modifications | O-glycosylated. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. Ubiquitinated in the presence of TRIM2 and UBE2D1. |
| Function | Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks. |
| Protein Name | Neurofilament Light PolypeptideNf-L68 Kda Neurofilament ProteinNeurofilament Triplet L Protein |
| Database Links | Reactome: R-HSA-438066Reactome: R-HSA-442982Reactome: R-HSA-5673001Reactome: R-HSA-9609736Reactome: R-HSA-9617324Reactome: R-HSA-9620244 |
| Cellular Localisation | Cell ProjectionAxonCytoplasmCytoskeleton |
| Alternative Protein Names | Neurofilament Light Polypeptide proteinNf-L protein68 Kda Neurofilament Protein proteinNeurofilament Triplet L Protein proteinNEFL proteinNF68 proteinNFL protein |
Information sourced from Uniprot.org