Human Neutrophil Elastase (Neutrophil Elastase) Antibody Pair (STJAP00897)
General Information
| Short Description | This Neutrophil Elastase antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R03-1S-2/R06-4K-2 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Purification |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | ELANE |
| Gene ID | 1991 |
| Uniprot ID | ELNE_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | Bone marrow cells. Neutrophil. |
| Function | Serine protease that modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. Promotes cleavage of GSDMB, thereby inhibiting pyroptosis. Promotes blood coagulation. Through the activation of the platelet fibrinogen receptor integrin alpha-IIb/beta-3, potentiates platelet aggregation induced by a threshold concentration of cathepsin G (CTSG). Cleaves and thus inactivates tissue factor pathway inhibitor (TFPI). Capable of killing E.coli but not S.aureus in vitro.digests outer membrane protein A (ompA) in E.coli and K.pneumoniae. |
| Protein Name | Neutrophil ElastaseBone Marrow Serine ProteaseElastase-2Human Leukocyte ElastaseHleMedullasinPmn Elastase |
| Database Links | Reactome: R-HSA-1442490Reactome: R-HSA-1474228Reactome: R-HSA-1592389Reactome: R-HSA-5620971Reactome: R-HSA-6798695Reactome: R-HSA-6803157Reactome: R-HSA-977606Reactome: R-HSA-9911233 |
| Cellular Localisation | Cytoplasmic VesiclePhagosomeLocalized In Phagolysosomes Following Ingestion Of EColi By Neutrophils |
| Alternative Antibody Pair Names | Neutrophil Elastase antibody pairBone Marrow Serine Protease antibody pairElastase-2 antibody pairHuman Leukocyte Elastase antibody pairHle antibody pairMedullasin antibody pairPmn Elastase antibody pairELANE antibody pairELA2 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human Neutrophil Elastase (Neutrophil Elastase) Antibody Pair (STJAP00897)
This antibody pair detects Human Neutrophil Elastase (UniProt P08246) and is validated for reactivity with human samples only.
Both antibodies are rabbit-derived monoclonal IgGs. The two clones are R03-1S-2 and R06-4K-2, and both were raised against a recombinant protein immunogen.
This pair is validated for use in ELISA. It is supplied as unconjugated antibodies, so the user is responsible for labeling the detection antibody or using a secondary detection system appropriate for their assay setup.
Both antibodies are formulated in 0.01M PBS at pH 7.2 and have been purified by affinity purification. No conjugation or tag is present on either component.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquot and store at -20°C, and avoid repeated freeze/thaw cycles to maintain activity.
Working concentrations and dilution ranges are to be determined by the end user and are not pre-specified for this product. Titration experiments are recommended to optimize performance for each specific assay and sample type.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.