Human Neurofilament (Neurofilament) Antibody Pair (STJAP00799)
General Information
| Short Description | This Neurofilament antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R92-8B-9/R11-1D-9 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | NEFH |
| Gene ID | 4744 |
| Uniprot ID | NFH_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Post Translational Modifications | There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. |
| Function | Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks. |
| Protein Name | Neurofilament Heavy PolypeptideNf-H200 Kda Neurofilament ProteinNeurofilament Triplet H Protein |
| Database Links | |
| Cellular Localisation | CytoplasmCytoskeletonCell ProjectionAxon |
| Alternative Antibody Pair Names | Neurofilament Heavy Polypeptide antibody pairNf-H antibody pair200 Kda Neurofilament Protein antibody pairNeurofilament Triplet H Protein antibody pairNEFH antibody pairKIAA0845 antibody pairNFH antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human Neurofilament (Neurofilament) Antibody Pair (STJAP00799)
This antibody pair detects human Neurofilament, corresponding to UniProt entry P12036. Reactivity has been confirmed for human samples only.
Both antibodies are rabbit monoclonal IgGs supplied unconjugated at 1 mg/mL in 0.01M PBS, pH 7.2. The clone IDs are R92-8B-9 and R11-1D-9, and both are affinity purified.
This pair is validated for use in ELISA. It has not been tested or validated for other assay formats based on the available product data.
Working dilutions are determined by the end user and are not pre-specified for this pair. Titration experiments are recommended to establish optimal concentrations for each antibody in the intended assay setup.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquot and store at -20°C, and avoid repeated freeze/thaw cycles to maintain activity.
Both antibodies in this pair were raised against a recombinant protein immunogen corresponding to human Neurofilament. No additional immunogen region information is specified in the product data.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.