This NCOA3 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human tissue homogenates, cell lysates or other biological fluids..
Applications
ELISA
Reactivity
Human
Sensitivity
0.117ng/mL
Detection Limit
0.312-20ng/mL
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Storage Instruction
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
tissue homogenates, cell lysates or other biological fluids.
Additional Info
Tissue Specificity
Widely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney.
Post Translational Modifications
Acetylated by CREBBP. Acetylation occurs in the RID domain, and disrupts the interaction with nuclear receptors and regulates its function. Methylated by CARM1. Phosphorylated by IKK complex. Regulated its function. Phosphorylation at Ser-601 by CK1 promotes coactivator function.
Function
Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit.
Protein Name
Nuclear Receptor Coactivator 3Ncoa-3ActrAmplified In Breast Cancer 1 ProteinAib-1Cbp-Interacting ProteinPcipClass E Basic Helix-Loop-Helix Protein 42Bhlhe42Receptor-Associated Coactivator 3Rac-3Steroid Receptor Coactivator Protein 3Src-3Thyroid Hormone Receptor Activator Molecule 1Tram-1
CytoplasmNucleusMainly Cytoplasmic And Weakly NuclearUpon Tnf Activation And Subsequent PhosphorylationIt Translocates From The Cytoplasm To The Nucleus
Alternative ELISA Names
Nuclear Receptor Coactivator 3 ELISA kitNcoa-3 ELISA kitActr ELISA kitAmplified In Breast Cancer 1 Protein ELISA kitAib-1 ELISA kitCbp-Interacting Protein ELISA kitPcip ELISA kitClass E Basic Helix-Loop-Helix Protein 42 ELISA kitBhlhe42 ELISA kitReceptor-Associated Coactivator 3 ELISA kitRac-3 ELISA kitSteroid Receptor Coactivator Protein 3 ELISA kitSrc-3 ELISA kitThyroid Hormone Receptor Activator Molecule 1 ELISA kitTram-1 ELISA kitNCOA3 ELISA kitAIB1 ELISA kitBHLHE42 ELISA kitRAC3 ELISA kitTRAM1 ELISA kit
