Human Natriuretic Peptides B (Natriuretic Peptides B) Antibody Pair (STJAP00683)
General Information
| Short Description | This Natriuretic Peptides B antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Mouse/Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | 3C9-A9/1B5-B9-H8 |
| Isotype | IgG1/IgG2b |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | ELISA 1:1000 |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | NPPB |
| Gene ID | 4879 |
| Uniprot ID | ANFB_HUMAN |
| Immunogen | A synthesized peptide derived from BNP |
Additional Info
| Tissue Specificity | Brain natriuretic peptide 32: Detected in the cardiac atria (at protein level). Detected in the kidney distal tubular cells (at protein level). |
| Post Translational Modifications | The precursor molecule is proteolytically cleaved by the endoproteases FURIN or CORIN at Arg-102 to produce brain natriuretic peptide 32 and NT-proBNP. This likely occurs after it has been secreted into the blood, either during circulation or in the target cells. CORIN also cleaves the precursor molecule at additional residues including Arg-99 and possibly Lys-105. In patients with heart failure, processing and degradation of natriuretic peptides B occurs but is delayed, possibly due to a decrease in enzyme level or activity of CORIN and DPP4. Brain natriuretic peptide 32: Undergoes further proteolytic cleavage by various proteases such as DPP4, MME and possibly FAP, to give rise to a variety of shorter peptides. Cleaved at Pro-104 by the prolyl endopeptidase FAP (seprase) activity (in vitro). Degraded by IDE. During IDE degradation, the resulting products initially increase the activation of NPR1 and can also stimulate NPR2 to produce cGMP before the fragments are completely degraded and inactivated by IDE (in vitro). O-glycosylated on at least seven residues. In cardiomyocytes, glycosylation at Thr-97 is essential for the stability and processing of the extracellular natriuretic peptides B. Glycosylation, especially at Thr-97, may also be important for brain natriuretic peptide 32 stability and/or extracellular distribution. Glycosylation at Thr-97 appears to inhibit FURIN- or CORIN-mediated proteolytic processing, at least in HEK293 cells. |
| Function | Brain natriuretic peptide 32: Cardiac hormone that plays a key role in mediating cardio-renal homeostasis. May also function as a paracrine antifibrotic factor in the heart. Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses. Involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Binds the clearance receptor NPR3. NT-proBNP: May affect cardio-renal homeostasis. Able to promote the production of cGMP although its potency is very low compared to brain natriuretic peptide 32. BNP(3-32): May have a role in cardio-renal homeostasis. Able to promote the production of cGMP. |
| Protein Name | Natriuretic Peptides BBrain Natriuretic Factor ProhormonePreprobnpProbnpGamma-Brain Natriuretic PeptideIso-Anp Cleaved Into - Nt-ProbnpNt-Pro-BnpNt-Probnp(1-76 - Probnp(3-108 - Brain Natriuretic Peptide 32Bnp(1-32Bnp-32Brain Natriuretic PeptideBnp - Bnp(1-30 - Bnp(1-29 - Bnp(1-28 - Bnp(2-31 - Bnp(3-32Des-Serpro-BnpProbnp(79-108 - Bnp(3-30 - Bnp(3-29 - Brain Natriuretic Peptide 29Bnp(4-32 - Bnp(4-31 - Bnp(4-30 - Bnp(4-29 - Bnp(4-27 - Bnp(5-32 - Bnp(5-31 - Bnp(5-29 |
| Database Links | |
| Cellular Localisation | Nt-Probnp: SecretedDetected In BloodProbnp(3-108): SecretedBrain Natriuretic Peptide 32: SecretedBnp(3-32): Secreted |
| Alternative Antibody Pair Names | Natriuretic Peptides B antibody pairBrain Natriuretic Factor Prohormone antibody pairPreprobnp antibody pairProbnp antibody pairGamma-Brain Natriuretic Peptide antibody pairIso-Anp Cleaved Into - Nt-Probnp antibody pairNt-Pro-Bnp antibody pairNt-Probnp(1-76 - Probnp(3-108 - Brain Natriuretic Peptide 32 antibody pairBnp(1-32 antibody pairBnp-32 antibody pairBrain Natriuretic Peptide antibody pairBnp - Bnp(1-30 - Bnp(1-29 - Bnp(1-28 - Bnp(2-31 - Bnp(3-32 antibody pairDes-Serpro-Bnp antibody pairProbnp(79-108 - Bnp(3-30 - Bnp(3-29 - Brain Natriuretic Peptide 29 antibody pairBnp(4-32 - Bnp(4-31 - Bnp(4-30 - Bnp(4-29 - Bnp(4-27 - Bnp(5-32 - Bnp(5-31 - Bnp(5-29 antibody pairNPPB antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human Natriuretic Peptides B (Natriuretic Peptides B) Antibody Pair (STJAP00683)
This antibody pair detects human Natriuretic Peptide B (BNP), corresponding to UniProt entry P16860. Reactivity has been confirmed for human samples only.
Both antibodies are mouse monoclonal IgGs supplied unconjugated in liquid format at 1 mg/mL. The capture antibody is clone 3C9-A9 (IgG1) and the detection antibody is clone 1B5-B9-H8 (IgG2b), both affinity purified.
This pair is validated for ELISA use, with a recommended dilution of 1:1000 for both antibodies.
Both antibodies were raised against a synthesized peptide derived from human BNP.
The antibodies should be stored at 4°C for short-term use. For long-term storage, aliquot and store at -20°C, and avoid repeated freeze/thaw cycles.
Both antibodies are supplied as a liquid formulation in PBS containing 50% glycerol, 0.5% BSA, and 0.02% sodium azide at pH 7.3.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.