Human NANOG protein (Recombinant) (N-His) (STJP006005)

SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
STJP006005
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General Information

Short DescriptionRecombinant-Human NANOG-N-His protein was developed from e.coli for the region N-His. For use in research applications.
ApplicationsELISA/Immunogen/SDS-PAGE/WB
HostE.coli
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

Dilution RangeReconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
FormulationLyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Storage InstructionUse a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt.

Target Information

Gene SymbolNANOG
Gene ID79923
Uniprot IDNANOG_HUMAN
ImmunogenHomo sapiens (Human)
Immunogen RegionTrp153-Val305

Additional Info

Function Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAATGTGT-3' or 5'-CGGACGCGCATTANGC-3'. Binds to the POU5F1/OCT4 promoter. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.
Protein Name Homeobox Protein Nanog
Homeobox Transcription Factor Nanog
Hnanog
Database Links Reactome: R-HSA-2892245
Reactome: R-HSA-2892247
Reactome: R-HSA-452723
Reactome: R-HSA-6785807
Reactome: R-HSA-9754189
Reactome: R-HSA-9823739
Reactome: R-HSA-9827857
Cellular Localisation Nucleus
Alternative Protein Names Homeobox Protein Nanog protein
Homeobox Transcription Factor Nanog protein
Hnanog protein
NANOG protein

Information sourced from Uniprot.org

Citations

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