Human N-Cadherin/CDH2 protein (Recombinant) (C-hFC) (STJP020098)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human N-Cadherin/CDH2 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asp160-Ala724) of Human N-Cadherin/CDH2 (Accession #NP_001783.2) fused with hFC at the C-terminus.
General Information
| Short Description | Recombinant-Human N-Cadherin/CDH2-C-hFC protein was developed in hek293 cells using the region Asp160-Ala724. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.2 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | < 0.01 EU/Mu g of the protein by LAL method |
Target Information
| Gene Symbol | CDH2 |
| Gene ID | 1000 |
| Uniprot ID | CADH2_HUMAN |
| Immunogen | Recombinant Human N-Cadherin/CDH2 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asp160-Ala724) of Human N-Cadherin/CDH2 (Accession #NP_001783.2) fused with hFC at the C-terminus. |
| Immunogen Region | Asp160-Ala724 |
| Immunogen Sequence | DWVIPPINLPENSRGPFPQE LVRIRSDRDKNLSLRYSVTG PGADQPPTGIFIINPISGQL SVTKPLDREQIARFHLRAHA VDINGNQVENPIDIVINVID MNDNRPEFLHQVWNGTVPEG SKPGTYVMTVTAIDADDPNA LNGMLRYRIVSQAPSTPSPN MFTINNETGDIITVAAGLDR EKVQQYTLIIQATDMEGNPT YGLSNTATAVITVTDVNDNP PEFTAMTFYGEVPENRVDI |
Additional Info
| Post Translational Modifications | Cleaved by MMP24. Ectodomain cleavage leads to the generation of a soluble 90 kDa N-terminal soluble fragment and a 45 kDa membrane-bound C-terminal fragment 1 (CTF1), which is further cleaved by gamma-secretase into a 35 kDa. Cleavage in neural stem cells by MMP24 affects CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate neural stem cell quiescence. May be phosphorylated by OBSCN. |
| Function | Calcium-dependent cell adhesion protein.preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. Plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem (NCS) cells, promoting the formation of processes by NCS cells. Required for proper neurite branching. Required for pre- and postsynaptic organization. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. |
| Protein Name | Cadherin-2Cdw325Neural CadherinN-CadherinCd Antigen Cd325 |
| Database Links | Reactome: R-HSA-381426Reactome: R-HSA-418990Reactome: R-HSA-525793Reactome: R-HSA-8957275Reactome: R-HSA-9926550 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinSarcolemmaCell JunctionCell SurfaceDesmosomeAdherens JunctionColocalizes With Tmem65 At The Intercalated Disk In CardiomyocytesColocalizes With Obscn At The Intercalated Disk And At Sarcolemma In Cardiomyocytes |
| Alternative Protein Names | Cadherin-2 proteinCdw325 proteinNeural Cadherin proteinN-Cadherin proteinCd Antigen Cd325 proteinCDH2 proteinCDHN proteinNCAD protein |
Information sourced from Uniprot.org