Human MYCBP (c-Myc-binding protein) CLIA Kit for Quantitative Detection
SPECIFICATIONS
General Information
| Short Description | This human MYCBP kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Applications | CLIA |
| Reactivity | Human |
| Sensitivity | 18.75pg/mL |
| Detection Limit | 31.25~2000pg/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Chemiluminescence |
Target Information
| Gene Symbol | MYCBP |
| Gene ID | 26292 |
| Uniprot ID | MYCBP_HUMAN |
| Specificity | This kit recognizes Human MYCBP in samples. No significant cross-reactivity or interference between Human MYCBP and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Tissue Specificity | Highly expressed in heart, placenta, pancreas, skeletal muscle and kidney. Also present at low levels in lung. |
| Function | May control the transcriptional activity of MYC. Stimulates the activation of E box-dependent transcription by MYC. |
| Protein Name | C-Myc-Binding ProteinAssociate Of Myc 1Amy-1 |
| Database Links | |
| Cellular Localisation | CytoplasmNucleusMitochondrionTranslocates Into The Nucleus In The S Phase Of The Cell Cycle Upon An Increase Of Myc ExpressionFound In The Mitochondria When Associated With Akap1 |
| Alternative CLIA Names | C-Myc-Binding Protein CLIA kitAssociate Of Myc 1 CLIA kitAmy-1 CLIA kitMYCBP CLIA kitAMY1 CLIA kit |
| Specificity | This kit recognizes Human MYCBP in samples. No significant cross-reactivity or interference between Human MYCBP and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 88-99 | 93 |
| EDTA plasma(n=8) | 95-109 | 101 |
| Cell culture media(n=8) | 90-103 | 97 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 110.39 | 218.14 | 946.18 | 109.08 | 200.75 | 992.51 |
| Standard deviation | 13.34 | 20.18 | 95.00 | 12.47 | 16.44 | 87.54 |
| CV (%) | 12.08 | 9.25 | 10.04 | 11.43 | 8.19 | 8.82 |