Human MUC5B (Mucin-5B) Sandwich ELISA Kit (STJE0003586)

Name: Human MUC5B (Mucin-5B) Sandwich ELISA Kit (STJE0003586)
SKU: STJE0003586
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

STJE0003586

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Short Description	This MUC5B Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Human
Sensitivity	0.38ng/mL
Detection Limit	0.63~40ng/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Gene Symbol	MUC5B
Gene ID	727897
Uniprot ID	MUC5B_HUMAN
Specificity	This kit recognizes Human MUC5B in samples. No significant cross-reactivity or interference between Human MUC5B and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Post Translational Modifications	Highly glycosylated. C-, N- and O-glycosylated. C-mannosylated in the Cys-rich subdomains probably on the first Trp residue of the WXXW motif. Highly O-glycosylated in the Ser/Thr-rich tandem repeat (TR) region. The repeat region is about 59% O-glycosylated with a high abundance of NeuAc(2)Hex(1)HexNac1-ol.
Function	Gel-forming mucin that is thought to contribute to the lubricating and viscoelastic properties of whole saliva and cervical mucus.
Protein Name	Mucin-5b Muc-5b Cervical Mucin High Molecular Weight Salivary Mucin Mg1 Mucin-5 Subtype B - Tracheobronchial Sublingual Gland Mucin
Database Links	Reactome: R-HSA-5083625 Reactome: R-HSA-5083632 Reactome: R-HSA-5083636 Reactome: R-HSA-5621480 Reactome: R-HSA-913709 Reactome: R-HSA-977068
Cellular Localisation	Secreted
Alternative ELISA Names	Mucin-5b ELISA kit Muc-5b ELISA kit Cervical Mucin ELISA kit High Molecular Weight Salivary Mucin Mg1 ELISA kit Mucin-5 Subtype B - Tracheobronchial ELISA kit Sublingual Gland Mucin ELISA kit MUC5B ELISA kit MUC5 ELISA kit
Specificity	This kit recognizes Human MUC5B in samples. No significant cross-reactivity or interference between Human MUC5B and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	1.92	6.00	14.33	1.79	5.85	14.62
Standard deviation	0.11	0.34	0.58	0.11	0.32	0.57
CV (%)	5.73	5.67	4.05	6.15	5.47	3.90