Human MUC5B (Mucin-5B) CLIA Kit for Quantitative Detection

Name: Human MUC5B (Mucin-5B) CLIA Kit for Quantitative Detection
SKU: STJC0000937
Availability: InStock

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SPECIFICATIONS

STJC0000937

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Short Description	This human MUC5B kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
Applications	CLIA
Reactivity	Human
Sensitivity	75pg/mL
Detection Limit	125~8000pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Chemiluminescence

Gene Symbol	MUC5B
Gene ID	727897
Uniprot ID	MUC5B_HUMAN
Specificity	This kit recognizes Human MUC5B in samples. No significant cross-reactivity or interference between Human MUC5B and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Tissue Specificity	Expressed on surface airway epithelia. Expressed mainly in mucous cells of submucosal glands of airway tissues. Highly expressed in the sublingual gland. Also found in submaxillary glands, endocervix, gall bladder, and pancreas.
Post Translational Modifications	Highly glycosylated. C-, N- and O-glycosylated. C-mannosylated in the Cys-rich subdomains probably on the first Trp residue of the WXXW motif. Highly O-glycosylated in the Ser/Thr-rich tandem repeat (TR) region. The repeat region is about 59% O-glycosylated with a high abundance of NeuAc(2)Hex(1)HexNac1-ol.
Function	Gel-forming mucin that is thought to contribute to the lubricating and viscoelastic properties of whole saliva and cervical mucus.
Protein Name	Mucin-5b Muc-5b Cervical Mucin High Molecular Weight Salivary Mucin Mg1 Mucin-5 Subtype B - Tracheobronchial Sublingual Gland Mucin
Database Links	Reactome: R-HSA-5083625 Reactome: R-HSA-5083632 Reactome: R-HSA-5083636 Reactome: R-HSA-5621480 Reactome: R-HSA-913709 Reactome: R-HSA-977068
Cellular Localisation	Secreted
Alternative CLIA Names	Mucin-5b CLIA kit Muc-5b CLIA kit Cervical Mucin CLIA kit High Molecular Weight Salivary Mucin Mg1 CLIA kit Mucin-5 Subtype B - Tracheobronchial CLIA kit Sublingual Gland Mucin CLIA kit MUC5B CLIA kit MUC5 CLIA kit
Specificity	This kit recognizes Human MUC5B in samples. No significant cross-reactivity or interference between Human MUC5B and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	373.50	1161.50	3146.62	354.73	1164.49	2991.28
Standard deviation	33.88	100.93	200.75	38.42	117.85	314.08
CV (%)	9.07	8.69	6.38	10.83	10.12	10.50