Human MMP-9 protein (Recombinant) (C-His) (STJP000879)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human MMP-9 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala20-Asp707 (Q279R) ) of human MMP-9/CLG4B (Accession #NP_004985.2) fused with a 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human MMP-9-C-His protein was developed in hek293 cells using the region Ala20-Asp707 Q279R. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | 1. Measured in a cell migration assay using A549 cells. 1 ng/mL of Recombinant Human MMP-9 can effectively induce A549 cells migration. |2. Recombinant Human MMP-9 Protein cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific ac |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | MMP9 |
| Gene ID | 4318 |
| Uniprot ID | MMP9_HUMAN |
| Immunogen | Recombinant Human MMP-9 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala20-Asp707 (Q279R) ) of human MMP-9/CLG4B (Accession #NP_004985.2) fused with a 6×His tag at the C-terminus. |
| Immunogen Region | Ala20-Asp707 Q279R |
| Immunogen Sequence | APRQRQSTLVLFPGDLRTNL TDRQLAEEYLYRYGYTRVAE MRGESKSLGPALLLLQKQLS LPETGELDSATLKAMRTPRC GVPDLGRFQTFEGDLKWHHH NITYWIQNYSEDLPRAVIDD AFARAFALWSAVTPLTFTRV YSRDADIVIQFGVAEHGDGY PFDGKDGLLAHAFPPGPGIQ GDAHFDDDELWSLGKGVVVP TRFGNADGAACHFPFIFEGR SYSACTTDGRSDGLPWCST |
Additional Info
| Tissue Specificity | Detected in neutrophils (at protein level). Produced by normal alveolar macrophages and granulocytes. |
| Post Translational Modifications | Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9. N- and O-glycosylated. |
| Function | Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves NINJ1 to generate the Secreted ninjurin-1 form. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. |
| Protein Name | Matrix Metalloproteinase-9Mmp-992 Kda Gelatinase92 Kda Type Iv CollagenaseGelatinase BGelb Cleaved Into - 67 Kda Matrix Metalloproteinase-9 - 82 Kda Matrix Metalloproteinase-9 |
| Database Links | Reactome: R-HSA-1433557Reactome: R-HSA-1442490Reactome: R-HSA-1474228Reactome: R-HSA-1592389Reactome: R-HSA-2022090Reactome: R-HSA-3928665Reactome: R-HSA-6785807Reactome: R-HSA-6798695Reactome: R-HSA-9009391 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular Matrix |
| Alternative Protein Names | Matrix Metalloproteinase-9 proteinMmp-9 protein92 Kda Gelatinase protein92 Kda Type Iv Collagenase proteinGelatinase B proteinGelb Cleaved Into - 67 Kda Matrix Metalloproteinase-9 - 82 Kda Matrix Metalloproteinase-9 proteinMMP9 proteinCLG4B protein |
Information sourced from Uniprot.org