Human MMP-3 protein (Active Recombinant) (C-His) (STJP000991)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human MMP-3 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Tyr18-Cys477) of human MMP-3 (Accession #NP_002413.1) fused with a 6×His tag at the C-terminus.
General Information
| Short Description | Active Recombinant-Human MMP-3-C-His protein was developed in hek293 cells using the region Tyr18-Cys477. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK (Dnp)-NH2. The specific activity is >500 pmol/min/Mu g. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | MMP3 |
| Gene ID | 4314 |
| Uniprot ID | MMP3_HUMAN |
| Immunogen | Recombinant Human MMP-3 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Tyr18-Cys477) of human MMP-3 (Accession #NP_002413.1) fused with a 6×His tag at the C-terminus. |
| Immunogen Region | Tyr18-Cys477 |
| Immunogen Sequence | YPLDGAARGEDTSMNLVQKY LENYYDLEKDVKQFVRRKDS GPVVKKIREMQKFLGLEVTG KLDSDTLEVMRKPRCGVPDV GHFRTFPGIPKWRKTHLTYR IVNYTPDLPKDAVDSAVEKA LKVWEEVTPLTFSRLYEGEA DIMISFAVREHGDFYPFDGP GNVLAHAYAPGPGINGDAHF DDDEQWTKDTTGTNLFLVAA HEIGHSLGLFHSANTEALMY PLYHSLTDLTRFRLSQDDI |
Additional Info
| Post Translational Modifications | Directly cleaved by HTRA2 to produce active form. |
| Function | Metalloproteinase with a rather broad substrate specificity that can degrade fibronectin, laminin, gelatins of type I, III, IV, and V.collagens III, IV, X, and IX, and cartilage proteoglycans. Activates different molecules including growth factors, plasminogen or other matrix metalloproteinases such as MMP9. Once released into the extracellular matrix (ECM), the inactive pro-enzyme is activated by the plasmin cascade signaling pathway. Also acts intracellularly. For example, in dopaminergic neurons, gets activated by the serine protease HTRA2 upon stress and plays a pivotal role in DA neuronal degeneration by mediating microglial activation and alpha-synuclein/SNCA cleavage. In addition, plays a role in immune response and possesses antiviral activity against various viruses such as vesicular stomatitis virus, influenza A virus (H1N1) and human herpes virus 1. Mechanistically, translocates from the cytoplasm into the cell nucleus upon virus infection to influence NF-kappa-B activities. |
| Protein Name | Stromelysin-1Sl-1Matrix Metalloproteinase-3Mmp-3Transin-1 |
| Database Links | Reactome: R-HSA-1442490Reactome: R-HSA-1474228Reactome: R-HSA-1592389Reactome: R-HSA-2022090Reactome: R-HSA-2179392Reactome: R-HSA-6785807Reactome: R-HSA-9009391 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular MatrixNucleusCytoplasm |
| Alternative Protein Names | Stromelysin-1 proteinSl-1 proteinMatrix Metalloproteinase-3 proteinMmp-3 proteinTransin-1 proteinMMP3 proteinSTMY1 protein |
Information sourced from Uniprot.org