| Host: |
HEK293 cells |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Human MMP-2-C-His protein was developed from hek293 cells and has a target region of C-His. For use in research applications. |
| Formulation: |
Lyophilized from a 0.2 Mu m filtered solution of 20mM TrisHCl, 150mM NaCl, pH7.5. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MMP2 |
| Gene ID: |
4313 |
| Uniprot ID: |
MMP2_HUMAN |
| Immunogen Region: |
Ala30-Cys660 |
| Immunogen: |
Recombinant Human MMP-2 Protein is produced by Human cells expression system. The target protein is expressed with sequence (Ala30-Cys660) of human MMP-2/72 kDa type IV collagenase (Accession #P08253) fused with a 6×His tag at the C-terminus. |
| Post Translational Modifications | Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro. The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3. |
| Function | Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14. PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels. Isoform 2: Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways. |
| Protein Name | 72 Kda Type Iv Collagenase72 Kda GelatinaseGelatinase AMatrix Metalloproteinase-2Mmp-2Tbe-1 Cleaved Into - Pex |
| Database Links | Reactome: R-HSA-1442490Reactome: R-HSA-1474228Reactome: R-HSA-1592389Reactome: R-HSA-381426Reactome: R-HSA-3928665Reactome: R-HSA-6785807Reactome: R-HSA-9009391 |
| Cellular Localisation | Isoform 1: SecretedExtracellular SpaceExtracellular MatrixMembraneNucleusColocalizes With Integrin Alphav/Beta3 At The Membrane Surface In Angiogenic Blood Vessels And MelanomasFound In MitochondriaAlong MicrofibrilsAnd In Nuclei Of CardiomyocytesIsoform 2: CytoplasmMitochondrion |
| Alternative Protein Names | 72 Kda Type Iv Collagenase protein72 Kda Gelatinase proteinGelatinase A proteinMatrix Metalloproteinase-2 proteinMmp-2 proteinTbe-1 Cleaved Into - Pex proteinMMP2 proteinCLG4A protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
