Human MMP-2 (72 Kda Type Iv Collagenase) Antibody Pair (STJAP00421)
General Information
| Short Description | This MMP-2 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rabbit/Goat |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | MMP2 |
| Gene ID | 4313 |
| Uniprot ID | MMP2_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human MMP-2 protien.Detection Immunogen: Recombinant Human MMP-2 protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro. The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3. |
| Function | Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14. PEX, the C-terminal non-catalytic fragment of MMP2, possesses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels. Isoform 2: Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways. |
| Protein Name | 72 Kda Type Iv Collagenase72 Kda GelatinaseGelatinase AMatrix Metalloproteinase-2Mmp-2Tbe-1 Cleaved Into - Pex |
| Database Links | Reactome: R-HSA-1442490Reactome: R-HSA-1474228Reactome: R-HSA-1592389Reactome: R-HSA-381426Reactome: R-HSA-3928665Reactome: R-HSA-6785807Reactome: R-HSA-9009391 |
| Cellular Localisation | Isoform 1: SecretedExtracellular SpaceExtracellular MatrixMembraneNucleusColocalizes With Integrin Alphav/Beta3 At The Membrane Surface In Angiogenic Blood Vessels And MelanomasFound In MitochondriaAlong MicrofibrilsAnd In Nuclei Of CardiomyocytesIsoform 2: CytoplasmMitochondrion |
| Alternative Antibody Pair Names | 72 Kda Type Iv Collagenase antibody pair72 Kda Gelatinase antibody pairGelatinase A antibody pairMatrix Metalloproteinase-2 antibody pairMmp-2 antibody pairTbe-1 Cleaved Into - Pex antibody pairMMP2 antibody pairCLG4A antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human MMP-2 (72 Kda Type Iv Collagenase) Antibody Pair (STJAP00421)
This antibody pair detects human MMP-2 (72 kDa Type IV Collagenase, UniProt P08253) and is validated for reactivity with human samples only. No cross-reactivity with other species has been documented in the provided product information.
The capture antibody is supplied unconjugated and the detection antibody is biotin-conjugated. Both are provided at a concentration of 0.5 mg/mL.
This pair is validated for use in ELISA. No other assay formats are listed in the product documentation.
The capture antibody is formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.5. The detection antibody is formulated in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol at pH 7.5.
This pair is intended for use with serum, plasma, and other biological fluids of human origin. It is for research use only and is not validated for diagnostic or therapeutic applications.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain reagent integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.