Human MMACHC protein (Recombinant) (His-Tag) (STJP017684)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human MMACHC-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.5 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% Glycerol, 1mM DTT |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MMACHC |
| Gene ID | 25974 |
| Uniprot ID | MMAC_HUMAN |
| Accession Number | NP_056321 |
| Immunogen | Human |
| Immunogen Region | 1-282aa |
| Immunogen Sequence |
Additional Info
| Tissue Specificity | Widely expressed. Expressed at higher level in fetal liver. Also expressed in spleen, lymph node, thymus and bone marrow. Weakly or not expressed in peripheral blood leukocytes. |
| Function | Cobalamin (vitamin B12) cytosolic chaperone that catalyzes the reductive decyanation of cyanocob(III)alamin (cyanocobalamin, CNCbl) to yield cob(II)alamin and cyanide, using FAD or FMN as cofactors and NADPH as cosubstrate. Cyanocobalamin constitutes the inactive form of vitamin B12 introduced from the diet, and is converted into the active cofactors methylcobalamin (MeCbl) involved in methionine biosynthesis, and 5'-deoxyadenosylcobalamin (AdoCbl) involved in the TCA cycle. Forms a complex with the lysosomal transporter ABCD4 and its chaperone LMBRD1, to transport cobalamin across the lysosomal membrane into the cytosol. The processing of cobalamin in the cytosol occurs in a multiprotein complex composed of at least MMACHC, MMADHC, MTRR (methionine synthase reductase) and MTR (methionine synthase) which may contribute to shuttle safely and efficiently cobalamin towards MTR in order to produce methionine. Also acts as a glutathione transferase by catalyzing the dealkylation of the alkylcob(III)alamins MeCbl and AdoCbl, using the thiolate of glutathione for nucleophilic displacement to generate cob(I)alamin and the corresponding glutathione thioether. The conversion of incoming MeCbl or AdoCbl into a common intermediate cob(I)alamin is necessary to meet the cellular needs for both cofactors. Cysteine and homocysteine cannot substitute for glutathione in this reaction. |
| Protein Name | Cyanocobalamin Reductase / Alkylcobalamin DealkylaseAlkylcobalamin -Glutathione S-AlkyltransferaseCblcCyanocobalamin ReductaseCyanide-EliminatingMethylmalonic Aciduria And Homocystinuria Type C ProteinMmachc |
| Database Links | Reactome: R-HSA-3359473Reactome: R-HSA-3359474Reactome: R-HSA-9759218 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative Protein Names | Cyanocobalamin Reductase / Alkylcobalamin Dealkylase proteinAlkylcobalamin -Glutathione S-Alkyltransferase proteinCblc proteinCyanocobalamin Reductase proteinCyanide-Eliminating proteinMethylmalonic Aciduria And Homocystinuria Type C Protein proteinMmachc proteinMMACHC protein |
Information sourced from Uniprot.org