Human MLLT3 protein (Recombinant) (N-His) (STJP015744)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human MLLT3-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | Please contact us for further information. |
Target Information
| Gene Symbol | MLLT3 |
| Gene ID | 4300 |
| Uniprot ID | AF9_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Met1-Thr148 |
Additional Info
| Function | Chromatin reader component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Specifically recognizes and binds acylated histone H3, with a preference for histone H3 that is crotonylated. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. Recognizes and binds histone H3 crotonylated at 'Lys-9' (H3K9cr), and with slightly lower affinity histone H3 crotonylated at 'Lys-18' (H3K18cr). Also recognizes and binds histone H3 acetylated and butyrylated at 'Lys-9' (H3K9ac and H3K9bu, respectively), but with lower affinity than crotonylated histone H3. In the SEC complex, MLLT3 is required to recruit the complex to crotonylated histones. Recruitment of the SEC complex to crotonylated histones promotes recruitment of DOT1L on active chromatin to deposit histone H3 'Lys-79' methylation (H3K79me). Plays a key role in hematopoietic stem cell (HSC) maintenance by preserving, rather than conferring, HSC stemness. Acts by binding to the transcription start site of active genes in HSCs and sustaining level of H3K79me2, probably by recruiting DOT1L. |
| Protein Name | Protein Af-9All1-Fused Gene From Chromosome 9 ProteinMyeloid/Lymphoid Or Mixed-Lineage Leukemia Translocated To Chromosome 3 ProteinYeats Domain-Containing Protein 3 |
| Database Links | Reactome: R-HSA-112382Reactome: R-HSA-674695Reactome: R-HSA-75955 |
| Cellular Localisation | NucleusChromosomeColocalizes With Acylated Histone H3Colocalizes With Histone H3 Crotonylated At 'Lys-18' (H3k18cr) |
| Alternative Protein Names | Protein Af-9 proteinAll1-Fused Gene From Chromosome 9 Protein proteinMyeloid/Lymphoid Or Mixed-Lineage Leukemia Translocated To Chromosome 3 Protein proteinYeats Domain-Containing Protein 3 proteinMLLT3 proteinAF9 proteinYEATS3 protein |
Information sourced from Uniprot.org