Human MLF1IP protein (Recombinant) (His-Tag) (STJP018595)

SPECIFICATIONS
HostE.coli
ImmunogenHuman
STJP018595
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General Information

Short DescriptionRecombinant-Human MLF1IP-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications.
ApplicationsSDS-PAGE/Denatured
HostE.coli
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

Concentration1 mg/mL
FormulationLiquid in 20mM Tris-HCl buffer (pH 8.0) containing 10% Glycerol, 0.4M urea
Storage InstructionFor short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolCENPU
Gene ID79682
Uniprot IDCENPU_HUMAN
Accession NumberNP_078905
ImmunogenHuman
Immunogen Region147-418a
Immunogen SequenceTRRKVKS AEKISTQRHE VIRTTASSEL SEKPAESVTS KKTGPLSAQP SVEKENLAIE SQSKTQKKGK ISHDKRKKSR SKAIGSDTSD IVHIWCPEGM KTSDIKELNI VLPEFEKTHL EHQQRIESKV CKAAIATFYV NVKEQFIKML KESQMLTNLK RKNAKMISDI EKKRQRMIEV QDELLRLEPQ LKQLQTKYDE LKERKS

Additional Info

Tissue Specificity Expressed at high levels in the testis, fetal liver, thymus, bone marrow and at lower levels in the lymph nodes, placenta, colon and spleen. Present in all cell lines examined, including B-cells, T-cells, epithelial cells and fibroblast cells. Expressed at high levels in glioblastoma cell lines.
Post Translational Modifications Phosphorylated by PLK1 at Thr-78, creating a self-tethering site that specifically interacts with the polo-box domain of PLK1.
Function Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Plays an important role in the correct PLK1 localization to the mitotic kinetochores. A scaffold protein responsible for the initial recruitment and maintenance of the kinetochore PLK1 population until its degradation. Involved in transcriptional repression.
Protein Name Centromere Protein U
Cenp-U
Centromere Protein Of 50 Kda
Cenp-50
Interphase Centromere Complex Protein 24
Kshv Latent Nuclear Antigen-Interacting Protein 1
Mlf1-Interacting Protein
Polo-Box-Interacting Protein 1
Database Links Reactome: R-HSA-141444
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-5663220
Reactome: R-HSA-606279
Reactome: R-HSA-68877
Reactome: R-HSA-9648025
Cellular Localisation Cytoplasm
Nucleus
Chromosome
Centromere
Kinetochore
Localizes In The Kinetochore Domain Of Centromeres
Colocalizes With Plk1 At The Interzone Between The Inner And The Outer Kinetochore Plates
Alternative Protein Names Centromere Protein U protein
Cenp-U protein
Centromere Protein Of 50 Kda protein
Cenp-50 protein
Interphase Centromere Complex Protein 24 protein
Kshv Latent Nuclear Antigen-Interacting Protein 1 protein
Mlf1-Interacting Protein protein
Polo-Box-Interacting Protein 1 protein
CENPU protein
ICEN24 protein
KLIP1 protein
MLF1IP protein
PBIP1 protein

Information sourced from Uniprot.org

Citations

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